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Development of Rat DC by in Vitro Culture of Bone Marrow Cells

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Dendritic Cells in Fundamental and Clinical Immunology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 378))

Abstract

Dendritic cells (DC) represent a subpopulation of leukocytes of bone marrow (BM) origin, involved in crucial immunological reactions. DC play a fundamental role in the primary immune response by stimulating quiescent T cells. In this study we describe an in vitro culture system to raise DC from unfractionated bone marrow (BM) cells of LEWIS rats in the presence of low doses of mouse recombinant GM-CSF, that was successfully used in previous work to culture mouse DC1,2,3.

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References

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Authors and Affiliations

  1. Institut für Immunologie, der Johannes Gutenberg-Universität, Obere Zahlbacher Straße 67, 55131, Mainz, Germany

    M. Mehlig, C. Scheicher & K. Reske

  2. Institut für Pathologie, Klinikum der Johannes Gutenberg-Universität, Obere Zahlbacher Straße 67, 55131, Mainz, Germany

    H.-P. Dienes

Authors
  1. M. Mehlig
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  2. C. Scheicher
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  3. H.-P. Dienes
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  4. K. Reske
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Editor information

Editors and Affiliations

  1. Laboratory for Immunological Research, Schering-Plough, Dardilly, France

    Jacques Banchereau

  2. INSERM U346, Hôpital Edouard Herriot, Lyon, France

    Daniel Schmitt

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© 1995 Springer Science+Business Media New York

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Mehlig, M., Scheicher, C., Dienes, HP., Reske, K. (1995). Development of Rat DC by in Vitro Culture of Bone Marrow Cells. In: Banchereau, J., Schmitt, D. (eds) Dendritic Cells in Fundamental and Clinical Immunology. Advances in Experimental Medicine and Biology, vol 378. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1971-3_14

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  • DOI: https://doi.org/10.1007/978-1-4615-1971-3_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5811-4

  • Online ISBN: 978-1-4615-1971-3

  • eBook Packages: Springer Book Archive

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