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Transgenic Models for Studies of Oxytocin and Vasopressin

  • Chapter
Transgenic Models in Endocrinology

Part of the book series: Endocrine Updates ((ENDO,volume 13))

Abstract

The number of studies of the nervous system using transgenic mice has grown explosively over the past decade, presenting a wide spectrum of approaches. Transgenic mice have been used to model human disease, to understand physiologic roles of genes, and to understand the regulation of genes (1). For example, transgenes composed of a gene’s promoter directing expression of a reporter gene such as beta-galactosidase may be used to follow expression of a gene during development (2), after various mutations are made in the promoter region to look for cell or regulatory specificity (3), and to study various physiological states (4). Regulatory studies may also use reporters (e.g., green fluorescent protein) that allow for real-time measurement of activity, either in vivo or in various tissue preparations, that is precluded by difficulties in assaying the promoter’s natural gene product. Transgenic mice may also be used to perturb a particular system by overexpressing a gene or by reducing the gene’s expression and/or effectiveness through antisense or dominant negative expression (5). Transgenic expression of certain products, such as tumor promoters or fluorescent substances, may allow for the isolation of immortalized and homogeneous cells for further study (6,7). Finally, transgenic mice may be used in the attempt to correct defects in mutant mice, either those found accidentally or those produced through homologous recombination or random mutagenesis (8).

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  1. National Institute of Mental Health, National Institutes of Health, Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, and the Section on Neural Gene Expression, Bethesda, MD, 20892, USA

    Harold Gainer & W. Scott Young III

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  1. Harold Gainer
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  1. The University of Manchester, UK

    Maria G. Castro Ph.D.

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Gainer, H., Young, W.S. (2001). Transgenic Models for Studies of Oxytocin and Vasopressin. In: Castro, M.G. (eds) Transgenic Models in Endocrinology. Endocrine Updates, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1633-0_2

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