Abstract
Integrons possess a site-specific recombination system and comprise a family of elements that are broadly distributed amongst the Proteobacteria. The units of capture into these elements are gene cassettes, which normally comprise of only a single gene along with an attachment site recognized by the recombination system. The class 1 integron has at least two features that distinguishes it from most other members of the integron family of integrase elements. The first of these is that they are located on mobile elements as opposed to being fixed in the chromosome and the second is that most of the associated gene cassettes include genes that encode antibiotic resistance. The linkage of the class 1 integron to mobile elements was an important step since it has meant that diverse molecular processes act cooperatively to disseminate resistance genes in Gram-negative bacteria. The selection for resistance in the antibiotic era has now led to an enormous diversity of elements that in many cases has resulted in conjugation, transposition, and site-specific recombination processes combining to spread large clusters of resistance genes. All these processes existed in nature prior to the antibiotic era but the level and extent of cooperation did not. Here we discuss how some of these complex class 1-associated mobile resistance regions evolved and their ramifications for the management of the antibiotic resistance problem.
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References
Lederberg J, Tatum EL (1946) Gene recombination in Escherichia coli. Nature 158:558
Hughes VM, Datta N (1983) Conjugative plasmids in bacteria of the ‘pre-antibiotic’ era. Nature 302:725–726
Gillings MR, Stokes HW (2012) Are humans increasing bacterial evolvability? Trends Ecol Evol 27:346–352
Hall RM, Brookes DE, Stokes HW (1991) Site-specific insertion of genes into integrons: role of the 59-base element and determination of the recombination cross-over point. Mol Microbiol 5:1941–1959
Martinez E, de la Cruz F (1988) Transposon Tn21 encodes a RecA-independent site-specific integration system. Mol Gen Genet 211:320–325
Recchia GD, Hall RM (1995) Gene cassettes: a new class of mobile element. Microbiology 141:3015–3027
Stokes HW, Hall RM (1989) A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons. Mol Microbiol 3:1669–1683
Partridge SR, Tsafnat G, Coiera E, Iredell JR (2009) Gene cassettes and cassette arrays in mobile resistance integrons. FEMS Microbiol Rev 33:757–784
Holzel CS, Harms KS, Bauer J, Bauer-Unkauf I, Hormansdorfer S, Kampf P et al (2012) Diversity of antimicrobial resistance genes and class-1-integrons in phylogenetically related porcine and human Escherichia coli. Vet Microbiol 160:403–412
Maguire AJ, Brown DF, Gray JJ, Desselberger U (2001) Rapid screening technique for class 1 integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology. Antimicrob Agents Chemother 45:1022–1029
Xu Z, Li L, Shi L, Shirtliff ME (2011) Class 1 integron in staphylococci. Mol Biol Rep 38:5261–5279
Brown H, Stokes H, Hall R (1996) The integrons In0, In2, and In5 are defective transposon derivatives. J Bacteriol 178:4429–4437
Kholodii GY, Mindlin SZ, Bass IA, Yurieva OV, Minakhina SV, Nikiforov VG (1995) Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integron. Mol Microbiol 17:1189–1200
Boucher Y, Labbate M, Koenig JE, Stokes HW (2007) Integrons: mobilizable platforms that promote genetic diversity in bacteria. Trends Microbiol 15:301–309
Mazel D (2006) Integrons: agents of bacterial evolution. Nat Rev Micro 4:608–620
Hansson K, Sundstrom L, Pelletier A, Roy PH (2002) IntI2 integron integrase in Tn7. J Bacteriol 184:1712–1721
Solberg OD, Ajiboye RM, Riley LW (2006) Origin of Class 1 and 2 Integrons and gene cassettes in a population-based sample of uropathogenic Escherichia coli. J Clin Microbiol 44:1347–1351
Fallah F, Karimi A, Goudarzi M, Shiva F, Navidinia M, Hadipour Jahromi M et al (2012) Jul 20) Determination of integron frequency by a polymerase chain reaction-restriction fragment length polymorphism method in multidrug-resistant Escherichia coli, which causes urinary tract infections. Microb Drug Resist 18(6):546–549
Mokracka J, Koczura R, Kaznowski A (2012) Multiresistant Enterobacteriaceae with class 1 and class 2 integrons in a municipal wastewater treatment plant. Water Res 46:3353–3363
Marquez C, Labbate M, Ingold AJ, Roy Chowdhury. P, Ramirez MS, Centron D et al (2008) Recovery of a functional class 2 integron from an Escherichia coli strain mediating a urinary tract infection. Antimicrob Agents Chemother 52:4153–4154
Barlow RS, Gobius KS (2006) Diverse class 2 integrons in bacteria from beef cattle sources. J Antimicrob Chemother 58:1133–1138
Arakawa Y, Murakami M, Suzuki K, Ito H, Wacharotayankun R, Ohsuka S et al (1995) A novel integron-like element carrying the metallo-β-lactamase gene blaIMP. Antimicrob Agents Chemother 39:1612–1615
Collis CM, Kim M-J, Partridge SR, Stokes HW, Hall RM (2002) Characterization of the class 3 integron and the site-specific recombination system it determines. J Bacteriol 184:3017–3026
Correia M, Boavida F, Grosso F, Salgado MJ, Lito LM, Cristino JM et al (2003) Molecular characterization of a new class 3 integron in Klebsiella pneumoniae. Antimicrob Agents Chemother 47:2838–2843
Shibata N, Doi Y, Yamane K, Yagi T, Kurokawa H, Shibayama K et al (2003) PCR typing of genetic determinants for metallo-β-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J Clin Microbiol 41:5407–5413
Xu H, Davies J, Miao V (2007) Molecular characterization of class 3 integrons from Delftia spp. J Bacteriol 189:6276–6283
Cambray G, Guerout AM, Mazel D (2010) Integrons. Annu Rev Genet 44:141–166
Gillings M, Boucher Y, Labbate M, Holmes A, Krishnan S, Holley M et al (2008) The evolution of class 1 integrons and the rise of antibiotic resistance. J Bacteriol 190:5095–510044141–166
Hall RM, Brown HJ, Brookes DE, Stokes HW (1994) Integrons found in different locations have identical 5′2 ends but variable 3′2 ends. J Bacteriol 176:6286–6294
Partridge SR, Hall RM (2004) Complex multiple antibiotic and mercury resistance region derived from the r-det of NR1 (R100). Antimicrob Agents Chemother 48:4250–4255
Partridge SR, Recchia GD, Stokes HW, Hall RM (2001) Family of class 1 integrons related to In4 from Tn1696. Antimicrob Agents Chemother 45:3014–3020
Partridge SR, Brown HJ, Hall RM (2002) Characterization and movement of the class 1 integron known as Tn2521 and Tn1405. Antimicrob Agents Chemother 46:1288–1294
Levesque C, Piche L, Larose C, Roy PH (1995) PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39:185–191
Radstrom P, Skold O, Swedberg G, Flensburg J, Roy PH, Sundstrom L (1994) Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements. J Bacteriol 176:3257–3268
Betteridge T, Partridge SR, Iredell JR, Stokes HW (2011) Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit. Antimicrob Agents Chemother 55:3939–3943
Dawes FE, Kuzevski A, Bettelheim KA, Hornitzky MA, Djordjevic SP, Walker MJ (2012) Distribution of class 1 integrons with IS26-mediated deletions in their 3′-conserved segments in Escherichia coli of human and animal origin. PLoS One 5:e12754
Tato M, Coque TM, Baquero F, Canton R (2012) Dispersal of carbapenemase blaVIM-1 gene associated with different Tn402 variants, mercury transposons, and conjugative plasmids in Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother 54:320–327
Toleman MA, Vinodh H, Sekar U, Kamat V, Walsh TR (2007) blaVIM-2-Harboring integrons isolated in India, Russia, and the United States arise from an ancestral class 1 integron predating the formation of the 3-conserved sequence. Antimicrob Agents Chemother 51:2636–2638
Gillings MR, Labbate M, Sajjad A, Giguere NJ, Holley MP, Stokes HW (2009) Mobilization of a Tn402-like class 1 integron with a novel cassette array via flanking miniature inverted-repeat transposable element-like structures. Appl Environ Microbiol 75:6002–6004
Marquez C, Labbate M, Raymondo C, Fernandez J, Gestal AM, Holley M et al (2008) Urinary tract infections in a South American population: dynamic spread of class 1 integrons and multidrug resistance by homologous and site-specific recombination. J Clin Microbiol 46:3417–3425
Partridge SR, Brown HJ, Stokes HW, Hall RM (2001) Transposons Tn1696 and Tn21 and their integrons In4 and In2 have independent origins. Antimicrob Agents Chemother 45:1263–1270
Toleman MA, Walsh TR (2010) ISCR elements are key players in IncA/C plasmid evolution. Antimicrob Agents Chemother 54:3534
Stokes HW, Nesbo CL, Holley M, Bahl MI, Gillings MR, Boucher Y (2006) Class 1 integrons potentially predating the association with Tn402-Like transposition genes are present in a sediment microbial community. J Bacteriol 188:5722–5730
Gillings MR, Xuejun D, Hardwick SA, Holley MP, Stokes HW (2009) Gene cassettes encoding resistance to quaternary ammonium compounds: a role in the origin of clinical class 1 integrons? ISME J 3:209–215
Liebert CA, Hall RM, Summers AO (1999) Transposon Tn21, Flagship of the floating genome. Microbiol Mol Biol Rev 63:507–522
Petrova M, Gorlenko Z, Mindlin S (2011) Tn5045, a novel integron-containing antibiotic and chromate resistance transposon isolated from a permafrost bacterium. Res Microbiol 162:337–345
Allmeier H, Cresnar B, Greck M, Schmitt R (1992) Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein. Gene 111:11–20
Poirel L, Decousser JW, Nordmann P (2003) Insertion sequence ISEcp1B is involved in expression and mobilization of a bla(CTX-M) beta-lactamase gene. Antimicrob Agents Chemother 47:2938–2945
Yamamoto T (1989) Organization of complex transposon Tn2610 carrying two copies of tnpA and tnpR. Antimicrob Agents Chemother 33:746–750
Rogowsky P, Schmitt R (1984) Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site. Mol Gen Genet 193:162–166
Zong Z, Yu R, Wang X, Lu X (2011) blaCTX-M-65 is carried by a Tn1722-like element on an IncN conjugative plasmid of ST131 Escherichia coli. J Medical Microbiol 60:435–441
Labbate M, Roy Chowdhury P, Stokes HW (2008) A class 1 integron present in a human commensal has a hybrid transposition module compared to Tn402: evidence of interaction with mobile DNA from natural environments. J Bacteriol 190:5318–5327
Juan C, Zamorano L, Mena A, Alberti S, Perez JL, Oliver A (2010) Metallo-β-lactamase-producing Pseudomonas putida as a reservoir of multidrug resistance elements that can be transferred to successful Pseudomonas aeruginosa clones. J Antimicrob Chemother 65:474–478
Marchiaro P, Viale AM, Ballerini V, Rossignol G, Vila AJ, Limansky A (2010) First report of a Tn402-like class 1 integron carrying blaVIM-2 in Pseudomonas putida from Argentina. J Infect Dev Ctries 4:412–416
Lagatolla C, Edalucci E, Dolzani L, Riccio ML, De Luca F, Medessi E et al (2006) Molecular evolution of metallo-β-lactamase-producing Pseudomonas aeruginosa in a nosocomial setting of high-level endemicity. J Clin Microbiol 44:2348–2353
Minakhina S, Kholodii G, Mindlin S, Yurieva O, Nikiforov V (1999) Tn5053 family transposons are res site hunters sensing plasmidal res sites occupied by cognate resolvases. Mol Microbiol 33:1059–1068
Roy Chowdhury P, Merlino J, Labbate M, Cheong EY, Gottlieb T, Stokes HW (2009) Tn6060, a transposon from a genomic island in a Pseudomonas aeruginosa clinical isolate that includes two class 1 integrons. Antimicrob Agents Chemother 53:5294–5296
Stokes HW, Elbourne LD, Hall RM (2007) Tn1403, a multiple-antibiotic resistance transposon made up of three distinct transposons. Antimicrob Agents Chemother 51:1827–1829
Martinez E, Marquez C, Ingold A, Merlino J, Djordjevic SP, Stokes HW et al (2012) Diverse mobilized class 1 integrons are common in the chromosomes of pathogenic Pseudomonas aeruginosa clinical isolates. Antimicrob. Agents Chemother 56:2169–2172
Roy Chowdhury P, Ingold A, Vanegas N, Martà nez E, Merlino J, Merkier AK et al (2011) Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study. Antimicrob Agents Chemother 55:3140–3149
Lartigue MF, Poirel L, Aubert D, Nordmann P (2006) In vitro analysis of ISEcp1B-mediated mobilization of naturally occurring beta-lactamase gene blaCTX-M of Kluyvera ascorbata. Antimicrob Agents Chemother 50:1282–1286
Olson AB, Silverman M, Boyd DA, McGeer A, Willey BM, Pong-Porter V et al (2005) Identification of a progenitor of the CTX-M-9 group of extended-spectrum β-lactamases from Kluyvera georgiana isolated in Guyana. Antimicrob Agents Chemother 49:2112–2115
Toleman MA, Bennett PM, Walsh TR (2006) ISCR elements: novel gene-capturing systems of the 21st century? Microbiol Mol Biol Rev 70:296–316
Stokes HW, Tomaras C, Parsons Y, Hall RM (1993) The partial 3′-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin. Plasmid 30:39–50
Szczepanowski R, Braun S, Riedel V, Schneiker S, Krahn I, Puhler A et al (2005) The 120 592 bp IncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions. Microbiology 151:1095–1111
Daly M, Villa L, Pezzella C, Fanning S, Carattoli A (2005) Comparison of multidrug resistance gene regions between two geographically unrelated Salmonella serotypes. J Antimicrob Chemother 55:558–561
Doublet B, Praud K, Weill FX, Cloeckaert A (2009) Association of IS26-composite transposons and complex In4-type integrons generates novel multidrug resistance loci in Salmonella genomic island 1. J Antimicrob Chemother 63:282–289
Espedido BA, Partridge SR, Iredell JR (2008) bla(IMP-4) in different genetic contexts in Enterobacteriaceae isolates from Australia. Antimicrob Agents Chemother 52:2984–2987
Domingues S, Nielsen KM, da Silva GJ (2011) The blaIMP-5-carrying integron in a clinical Acinetobacter baumannii strain is flanked by miniature inverted-repeat transposable elements (MITEs). J Antimicrob Chemother 66:2667–2668
Delihas N (2008) Small mobile sequences in bacteria display diverse structure/function motifs. Mol Microbiol 67:475–481
Delihas N (2007) Enterobacterial small mobile sequences carry open reading frames and are found intragenically-evolutionary implications for formation of new peptides. Gene Regul Syst Bio 1:191–205
Venturini C, Beatson SA, Djordjevic SP, Walker MJ (2010) Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid. FASEB J 24:1160–1166
Pan JC, Ye R, Wang HQ, Xiang HQ, Zhang W, Yu XF et al (2008) Vibrio cholerae O139 multiple-drug resistance mediated by Yersinia pestis pIP1202-like conjugative plasmids. Antimicrob Agents Chemother 52:3829–3836
Cain AK, Hall RM (2012) Evolution of a multiple antibiotic resistance region in IncHI1 plasmids: reshaping resistance regions in situ. J Antimicrob Chemother 67(12):2848–2853
Cain AK, Hall RM (2012) Evolution of IncHI2 plasmids via acquisition of transposons carrying antibiotic resistance determinants. J Antimicrob Chemother 67:1121–1127
Partridge SR, Ellem JA, Tetu SG, Zong Z, Paulsen IT, Iredell JR (2011) Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 isolate. Antimicrob Agents Chemother 55:5933–5935
Partridge SR, Paulsen IT, Iredell JR (2012) pJIE137 carrying blaCTX-M-62 is closely related to p271A carrying blaNDM-1. Antimicrob Agents Chemother 56:2166–2168
Chen YT, Liao TL, Liu YM, Lauderdale TL, Yan JJ, Tsai SF (2009) Mobilization of qnrB2 and ISCR1 in plasmids. Antimicrob Agents Chemother 53:1235–1237
Haines AS, Jones K, Batt SM, Kosheleva IA, Thomas CM (2007) Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny. Plasmid 58:76–83
Bashir A, Klammer AA, Robins WP, Chin CS, Webster D, Paxinos E et al (2012) A hybrid approach for the automated finishing of bacterial genomes. Nat Biotechnol 30(7):701–707
Hacker J, Kaper JB (2000) Pathogenicity islands and the evolution of microbes. Annu Rev Microbiol 54:641–679
Dobrindt U, Hochhut B, Hentschel U, Hacker J (2004) Genomic islands in pathogenic and environmental microorganisms. Nat Rev Micro 2:414–424
Fournier P-E, Vallenet D, Barbe V, Audic S, Ogata H, Poirel L et al (2006) Comparative genomics of multidrug resistance in Acinetobacter baumannii. PLoS Genet 2:e7
Lescat M, Calteau A, Hoede C, Barbe V, Touchon M, Rocha E et al (2009) A module located at a chromosomal integration hot spot is responsible for the multidrug resistance of a reference strain from Escherichia coli clonal group A. Antimicrob Agents Chemother 53:2283–2288
Klockgether J, Reva O, Larbig K, Tümmler B (2004) Sequence analysis of the mobile genome island pKLC102 of Pseudomonas aeruginosa. C J Bacteriol 186:518–534
Boyd D, Cloeckaert A, Chaslus-Dancla E, Mulvey MR (2002) Characterization of variant Salmonella genomic island 1 multidrug resistance regions from serovars Typhimurium DT104 and Agona. Antimicrob Agents Chemother 46:1714–1722
Klockgether J, Cramer N, Wiehlmann L, Davenport CF, Tummler B (2011) Pseudomonas aeruginosa genomic structure and diversity. Front Microbiol 2:1–18
Klockgether J, Wurdemann D, Reva O, Wiehlmann L, Tummler B (2006) Diversity of the abundant pKLC102/PAGI-2 family of genomic islands in Pseudomonas aeruginosa. J Bacteriol 6:2443–2459
Threlfall EJ, Ward LR, Frost JA, Willshaw GA (2000) The emergence and spread of antibiotic resistance in food-borne bacteria. Int J Food Microbiol 62:1–5
Zhao S, Blickenstaff K, Bodeis-Jones S, Gaines SA, Tong E, McDermott PF (2012) Comparison of the prevalences and antimicrobial resistances of Escherichia coli isolates from different retail meats in the United States, 2002 to 2008. Appl Environ Microbiol 78:1701–1707
Schwaiger K, Huther S, Holzel C, Kampf P, Bauer J (2012) Prevalence of antibiotic-resistant Enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany. Int J Food Microbiol 154:206–211
Buchholz U, Bernard H, Werber D, Bohmer MM, Remschmidt C, Wilking H et al (2011) German outbreak of Escherichia coli O104:H4 associated with sprouts. N Engl J Med 365:1763–1770
Mellmann A, Harmsen D, Cummings CA, Zentz EB, Leopold SR, Rico A et al (2011) Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLoS One 6:e22751
Bielaszewska M, Mellmann A, Zhang W, Kock R, Fruth A, Bauwens A et al (2011) Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect Dis 11:671–676
Leverstein-van Hall MA, M Blok HE, T Donders AR, Paauw A, Fluit AC, Verhoef J (2003) Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. J Infect Dis 187:251–259
Doublet B, Boyd D, Mulvey MR, Cloeckaert A (2005) The Salmonella genomic island 1 is an integrative mobilizable element. Mol Microbiol 55:1911–1924
Boyd D, Peters GA, Cloeckaert A, Boumedine KS, Chaslus-Dancla E, Imberechts H et al (2001) Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona. J Bacteriol 183:5725–5732
Mulvey MR, Boyd DA, Olson AB, Doublet B, Cloeckaert A (2006) The genetics of Salmonella genomic island 1. Microbes Infect 8:1915–1922
Threlfall EJ (2000) Epidemic Salmonella typhimurium DT 104–a truly international multiresistant clone. J Antimicrob Chemother 46:7–10
Levings RS, Lightfoot D, Partridge SR, Hall RM, Djordjevic SP (2005) The genomic island SGI1, containing the multiple antibiotic resistance region of Salmonella enterica serovar Typhimurium DT104 or variants of it, is widely distributed in other S. enterica serovars. J Bacteriol 187:4401–4409
Evans S, Davies R (1996) Case control study of multiple-resistant Salmonella typhimurium DT104 infection of cattle in Great Britain. Vet Rec 139:557–558
Wall PG, Morgan D, Lamden K, Ryan M, Griffin M, Threlfall EJ et al (1994) A case control study of infection with an epidemic strain of multiresistant Salmonella typhimurium DT104 in England and Wales. Commun Dis Rep CDR Rev 4:R130–5
Kiss J, Nagy B, Olasz F (2012) Stability, entrapment and variant formation of Salmonella genomic island 1. PLoS One 7:e32497
Le Hello S, Weill FX, Guibert V, Praud K, Cloeckaert A, Doublet B (2012) Early multidrug-resistant Salmonella enterica Serovar Kentucky ST198 from Southeast Asia harbor Salmonella genomic island 1-J variants with a novel insertion sequence. Antimicrob Agents Chemother 56(10):5096–5102
Djordjevic SP, Cain AK, Evershed NJ, Falconer L, Levings RS, Lightfoot D et al (2009) Emergence and evolution of multiply antibiotic-resistant Salmonella enterica serovar Paratyphi B D-tartrate-utilizing strains containing SGI1. Antimicrob Agents Chemother 53:2319–2326
Levings RS, Lightfoot D, Hall RM, Djordjevic SP (2006) Aquariums as reservoirs for multidrug-resistant Salmonella Paratyphi B. Emerg Infect Dis 12:507–510
Le Hello S, Hendriksen RS, Doublet B, Fisher I, Nielsen EM, Whichard JM et al (2011) International spread of an epidemic population of Salmonella enterica serotype Kentucky ST198 resistant to ciprofloxacin. J Infect Dis 204:675–684
Levings RS, Partridge SR, Djordjevic SP, Hall RM (2007) SGI1-K, a variant of the SGI1 genomic island carrying a mercury resistance region, in Salmonella enterica serovar Kentucky. Antimicrob Agents Chemother 51:317–323
Doublet B, Butaye P, Imberechts H, Boyd D, Mulvey MR, Chaslus-Dancla E et al (2004) Salmonella genomic island 1 multidrug resistance gene clusters in Salmonella enterica serovar Agona isolated in Belgium in 1992 to 2002. Antimicrob Agents Chemother 48:2510–2517
Doublet B, Butaye P, Imberechts H, Collard JM, Chaslus-Dancla E, Cloeckaert A (2004) Salmonella agona harboring genomic island 1-A. Emerg Infect Dis 10:756–758
Cain AK, Liu X, Djordjevic SP, Hall RM (2010) Transposons related to Tn1696 in IncHI2 plasmids in multiply antibiotic resistant Salmonella enterica serovar Typhimurium from Australian animals. Microb Drug Resist 16:197–202
Johnson TJ, Lang KS (2012) IncA/C plasmids: an emerging threat to human and animal health? Mob Genet Elements 2:55–58
Fricke WF, Welch TJ, McDermott PF, Mammel MK, LeClerc JE, White DG et al (2009) Comparative genomics of the IncA/C multidrug resistance plasmid family. J Bacteriol 191:4750–4757
Lindsey RL, Fedorka-Cray PJ, Frye JG, Meinersmann RJ (2009) Inc A/C plasmids are prevalent in multidrug-resistant Salmonella enterica isolates. Appl Environ Microbiol 75:1908–1915
Allen KJ, Poppe C (2002) Occurrence and characterization of resistance to extended-spectrum cephalosporins mediated by beta-lactamase CMY-2 in Salmonella isolated from food-producing animals in Canada. Can J Vet Res 66:137–144
Garcia P, Guerra B, Bances M, Mendoza MC, Rodicio MR (2011) IncA/C plasmids mediate antimicrobial resistance linked to virulence genes in the Spanish clone of the emerging Salmonella enterica serotype 4,[5],12:i. J Antimicrob Chemother 66:543–549
Doublet B, Boyd D, Douard G, Praud K, Cloeckaert A, Mulvey MR (2012) Complete nucleotide sequence of the multidrug resistance IncA/C plasmid pR55 from Klebsiella pneumoniae isolated in 1969. J Antimicrob Chemother 67(10):2354–2360
Saidani M, Hammami S, Kammoun A, Slim A, Boutiba-Ben Boubaker I (2012) Emergence of carbapenem resistant Enterobacteriaceae producing OXA-48 carbapenemase in Tunisia. J Med Microbiol 61(Pt 12):1746–1749
Galimand M, Guiyoule A, Gerbaud G, Rasoamanana B, Chanteau S, Carniel E et al (1997) Multidrug resistance in Yersinia pestis mediated by a transferable plasmid. N Engl J Med 337:677–680
Pan JC, Ye R, Wang HQ, Xiang HQ, Zhang W, Yu XF et al (2008) Vibrio cholerae O139 multiple-drug resistance mediated by Yersinia pestis pIP1202-like conjugative plasmids. Antimicrob Agents Chemother 52:3829–3836
Kim MJ, Hirono I, Kurokawa K, Maki T, Hawke J, Kondo H et al (2008) Complete DNA sequence and analysis of the transferable multiple-drug resistance plasmids (R Plasmids) from Photobacterium damselae subsp. piscicida isolates collected in Japan and the United States. Antimicrob Agents Chemother 52:606–611
Reith ME, Singh RK, Curtis B, Boyd JM, Bouevitch A, Kimball J et al (2008) The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into the evolution of a fish pathogen. BMC Genomics 9:427
Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R et al (2010) Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 10:597–602
Hopkins KL, Liebana E, Villa L, Batchelor M, Threlfall EJ, Carattoli A (2006) Replicon typing of plasmids carrying CTX-M or CMY beta-lactamases circulating among Salmonella and Escherichia coli isolates. Antimicrob Agents Chemother 50:3203–3206
Carattoli A, Miriagou V, Bertini A, Loli A, Colinon C, Villa L et al (2006) Replicon typing of plasmids encoding resistance to newer beta-lactams. Emerg Infect Dis 12:1145–1148.
Welch TJ, Fricke WF, McDermott PF, White DG, Rosso ML, Rasko DA et al (2007) Multiple antimicrobial resistance in plague: an emerging public health risk. PLoS One 2:e309
Switt AI, Soyer Y, Warnick LD, Wiedmann M (2009) Emergence, distribution, and molecular and phenotypic characteristics of Salmonella enterica serotype 4,5,12:i. Foodborne Pathog Dis 6:407–415
Hopkins KL, Kirchner M, Guerra B, Granier SA, Lucarelli C, Porrero MC et al (2010) Multiresistant Salmonella enterica serovar 4,[5],12:i:- in Europe: a new pandemic strain? Euro Surveill 15:19580
Lucarelli C, Dionisi AM, Torpdahl M, Villa L, Graziani C, Hopkins K et al (2010) Evidence for a second genomic island conferring multidrug resistance in a clonal group of strains of Salmonella enterica serovar Typhimurium and its monophasic variant circulating in Italy, Denmark, and the United Kingdom. J Clin Microbiol 48:2103–2109
Sarmah AK, Meyer MT, Boxall AB (2006) A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere 65:725–759
Heuer H, Schmitt H, Smalla K (2011) Antibiotic resistance gene spread due to manure application on agricultural fields. Curr Opin Microbiol 14:236–243
Heuer H, Smalla K (2007) Manure and sulfadiazine synergistically increased bacterial antibiotic resistance in soil over at least two months. Environ Microbiol 9:657–666
Binh CT, Heuer H, Gomes NC, Kotzerke A, Fulle M, Wilke BM et al (2007) Short-term effects of amoxicillin on bacterial communities in manured soil. FEMS Microbiol Ecol 62:290–302
Heuer H, Solehati Q, Zimmerling U, Kleineidam K, Schloter M, Muller T et al (2011) Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine. Appl Environ Microbiol 77:2527–2530
Binh CT, Heuer H, Kaupenjohann M, Smalla K (2008) Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids. FEMS Microbiol Ecol 66:25–37
Binh CT, Heuer H, Kaupenjohann M, Smalla K (2009) Diverse aadA gene cassettes on class 1 integrons introduced into soil via spread manure. Res Microbiol 160:427–433
Heuer H, Binh CT, Jechalke S, Kopmann C, Zimmerling U, Krogerrecklenfort E et al (2012) IncP-1epsilon plasmids are important vectors of antibiotic resistance genes in agricultural systems: diversification driven by class 1 integron gene cassettes. Front Microbiol 3:2
Tsafnat G, Copty J, Partridge SR. (2011) RAC: Repository of antibiotic resistance cassettes. Database : the journal of biological databases and curation. 2011:bar054.
Fluit AC, Schmitz FJ (1999) Class 1 integrons, gene cassettes, mobility, and epidemiology. Euro J Clin Microbiol Infect Dis 18:761–770
Rowe-Magnus DA, Mazel D (2002) The role of integrons in antibiotic resistance gene capture. J Med Microbiol 292:115–125
Fluit AC, Schmitz FJ (2004) Resistance integrons and super-integrons. Clin Microbiol Infect 10:272–288
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Martinez, E., Djordjevic, S., Stokes, H., Roy Chowdhury, P. (2013). Mobilized Integrons: Team Players in the Spread of Antibiotic Resistance Genes. In: Gophna, U. (eds) Lateral Gene Transfer in Evolution. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7780-8_4
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