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Antioxidant and DNA Protection Effects of Taurine by Electron Spin Resonance Spectroscopy

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Taurine 8

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 776))

Abstract

Taurine may play an important role in protecting cells against toxic injury by an antioxidant. However, there is a lack of evidence to support this hypothesis. The objective of this study was to examine the in vitro antioxidant properties of taurine against different reactive species at various concentrations. The radical scavenging effects of taurine on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide radical, and alkyl radical were investigated using a spin-trapping electron method and compared with the electron spin resonance (ESR) signal intensity. ESR assays showed that DPPH radical scavenging activity of taurine at various concentrations (0.0625 ∼ 1 mg/mL) was elevated with a decrease of ESR signals in a dose-dependent manner. Moreover, taurine exhibited the radical scavenging activities against hydroxyl radicals, superoxide radicals, and alkyl radicals. Findings from this study suggest that taurine may be a useful radical scavenger and a potential supplement for the food, pharmaceutical, and cosmetic industries as well as feed and/or antibiotic because of its potent antioxidant capacities against various reactive radicals.

Sun Hee Cheong and Sang Ho Moon contributed equally to this work.

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Abbreviations

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

ESR:

Electron spin resonance

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Acknowledgements

This work was carried out with the support of the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ907038)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Applied Biological Chemistry, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Sun Hee Cheong

  2. Department of Food and Nutrition, Inha University, Incheon, 402-751, South Korea

    Sun Hee Cheong & Kyung Ja Chang

  3. Department of Food Bio Science, College of Natural Sciences, Konkuk University, Chungju, Chungchongbuk-do, 380-701, South Korea

    Sang Ho Moon

  4. Department of Biotechnology, Division of Life Science, Konkuk University, College of Biomedical and Health Science, Chungju, Chungchongbuk-do, 380-701, South Korea

    Seung Jae Lee

  5. Department of Chemistry, Konkuk University, Seoul, 143-701, South Korea

    Sung Hoon Kim

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  1. Sun Hee Cheong
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Correspondence to Sun Hee Cheong .

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Editors and Affiliations

  1. City University of New York, Center for Developmental Neuroscience, College of Staten Island, 2800 Victory Blvd, Staten Island, 10314, New York, USA

    Abdeslem El Idrissi

  2. City University of New York, Department of Biology, College of Staten Island, Victory Blvd 2800, Staten Island, 10314, New York, USA

    William J. L'Amoreaux

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Cheong, S.H., Moon, S.H., Lee, S.J., Kim, S.H., Chang, K.J. (2013). Antioxidant and DNA Protection Effects of Taurine by Electron Spin Resonance Spectroscopy. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 776. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6093-0_17

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