Abstract
Before the umbilical blood is frozen, it will first be introduced to a solution to help prevent it from being damaged while frozen. This solution is referred to as the cryopreservation solvent or cryoprotectant [1]. Once the blood has received this, it will begin to slowly freeze. Freezing it gradually is used as another preventative measure in guarding the cells against damage. Once the blood is frozen to a temperature of −96°C, it is transferred to a permanent storage freezer where it will remain frozen through the use of either liquid or vapor nitrogen.
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References
Rubinstein P, Dobrila L, Rosenfield RE, Adamson JW, Migliaccio G, Migliaccio AR, Taylor PE, Stevens CE (1995) Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. Proc Natl Acad Sci USA 92(22):10119–10122
Reboredo NM, Díaz A, Castro A, Villaescusa RG (2000) Collection, processing and cryopreservation of umbilical cord blood for unrelated transplantation. BMT 26(12):1263–1270
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Pavlovic, M., Balint, B. (2013). Cord Blood Cell Cryopreservation. In: Stem Cells and Tissue Engineering. SpringerBriefs in Electrical and Computer Engineering. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5505-9_14
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DOI: https://doi.org/10.1007/978-1-4614-5505-9_14
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