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Principles and Practice of Stem Cell Cryopreservation

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Stem Cells and Tissue Engineering

Part of the book series: SpringerBriefs in Electrical and Computer Engineering ((BRIEFSELECTRIC))

Abstract

Recent extensive application of various cell-mediated therapeutic approaches has resulted in increased needs for both specific blood-derived cells and operating procedures to get minimized cell damages during their collection, processing, and storage in liquid or frozen state. The aim of cryoinvestigations is to minimize cell injuries during the freeze/thaw process (cryoinjury). Cryoinjuries may be the result of extensive cell dehydration and/or intracellular ice crystallization. The basic goal of cryopreservation is to maintain the cell viability—which may be defined as the ability of cells to perform their normal or near-normal function when transfused or transplanted. Generally, postthaw cell recovery is superior when the most appropriate freezing procedure and the best cryoprotective agent (cryoprotectant) are used. For blood progenitor or cell cryopreservation, glycerol, dimethyl sulfoxide (DMSO), and hydroxyetilstarch (HES) are regularly used, although in different concentrations. Despite the fact that cell freezing practice is already in routine use, some questions related to the optimal living cell cryopreservation are still unresolved.

“Problems worthy of attack prove their worth by fighting back.”

– Paul Erdos

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Authors and Affiliations

  1. Florida Atlantic University, Boca Raton, FL, USA

    Mirjana Pavlovic

  2. Military Medical Academy, Belgrade, Serbia

    Bela Balint

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  1. Mirjana Pavlovic
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  2. Bela Balint
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Pavlovic, M., Balint, B. (2013). Principles and Practice of Stem Cell Cryopreservation. In: Stem Cells and Tissue Engineering. SpringerBriefs in Electrical and Computer Engineering. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5505-9_13

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  • DOI: https://doi.org/10.1007/978-1-4614-5505-9_13

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-5504-2

  • Online ISBN: 978-1-4614-5505-9

  • eBook Packages: EngineeringEngineering (R0)

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