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Functional Assessment of Microbial and Viral Infections by Real-Time Cellular Analysis System

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Advanced Techniques in Diagnostic Microbiology

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Abstract

Microbes and viruses infect their host cells and in doing so alter the physiological functions of the host cells leading to diseases. Analysis of pathogen-infected cells provides critical information with regards to functional assessment of microbe–host and virus–host interactions in addition to serving as an important diagnosis for infection. As early as 1950s, hemagglutination assay using red blood cells (RBC) were employed to detect the presence of hemagglutinin antigen-bearing virus particles [1]. Recently this assay was also used for bacterial detection [2]. Some viral families and many bacteria have envelope or surface proteins, which are able to agglutinate human or animal RBC and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice structure will form, allowing for visual inspection.

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Author information

Authors and Affiliations

  1. ACEA Biosciences, Inc., 6779 Mesa Ridge Rd. #100, San Diego, CA, 92121, USA

    Xiao Xu M.D., Ph.D.

  2. State Key Laboratory of Diagnostic and Treatment for Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang, PR, 310006, China

    Min Zheng

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  1. Xiao Xu M.D., Ph.D.
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  2. Min Zheng
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Correspondence to Xiao Xu M.D., Ph.D. .

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Editors and Affiliations

  1. Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, 10065, New York, USA

    Yi-Wei Tang

  2. Vanderbilt Clinic, Clinical Microbiology Laboratory, Vanderbilt University Medical Center, 22nd Avenue 1301, Nashville, 37232-5310, Tennessee, USA

    Charles W. Stratton

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Xu, X., Zheng, M. (2013). Functional Assessment of Microbial and Viral Infections by Real-Time Cellular Analysis System. In: Tang, YW., Stratton, C. (eds) Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-3970-7_8

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