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Probe Amplification Technologies

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Advanced Techniques in Diagnostic Microbiology

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Abstract

Oligonucleotide probes provide a useful tool for the detection of target nucleic acids by the formation of a double-helical structure between complementary sequences. The stringent requirements of Watson–Crick base pairing make hybridization extremely specific. However, the detection of target sequence by hybridization is often insensitive due to the limited number of signal molecules that can be labeled on the probe. In general, the analytical sensitivity of probe hybridization is in the order of 106 molecules. Therefore, it cannot meet the needs of most clinical diagnostic applications. Many technologies have been developed to improve the detection sensitivity by amplifying the probe sequence bound to the target. All probe amplification technologies are developed based on the recent advancement in molecular biology and the understanding of in vivo nucleic acid synthesis, i.e., ligation, polymerization, transcription, digestion/cleavage, etc.

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Author information

Authors and Affiliations

  1. Molecular Pathology Laboratory, Department of Pathology, Mount Sinai School of Medicine, New York University, One Gustave Levy Place, 1122, New York, NY, 10029, USA

    Fei Ye, Miao Cui, Tao Feng, Ivy Lee, Josephine Wu, Bingjiao Yin & David Zhang

Authors
  1. Fei Ye
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  2. Miao Cui
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  3. Tao Feng
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  4. Ivy Lee
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  5. Josephine Wu
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  6. Bingjiao Yin
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  7. David Zhang
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Correspondence to David Zhang .

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Editors and Affiliations

  1. Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, 10065, New York, USA

    Yi-Wei Tang

  2. Vanderbilt Clinic, Clinical Microbiology Laboratory, Vanderbilt University Medical Center, 22nd Avenue 1301, Nashville, 37232-5310, Tennessee, USA

    Charles W. Stratton

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Ye, F. et al. (2013). Probe Amplification Technologies. In: Tang, YW., Stratton, C. (eds) Advanced Techniques in Diagnostic Microbiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-3970-7_17

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