Abstract
The intercellular space mobile tissue fluid percolating cells contains all the information which proteins, carbohydrates, and lipids were filtered from plasma and what cells consume and excrete, as well as disintegrated cell debris. Moreover, it contains immune cells playing the immunosurveillance role. Harvesting of this fluid, that is called lymph, once it flows into lymphatics, gives insight into all tissue events. We worked out a microsurgical method of cannulation human leg lymphatics for continuous collection of lymph. This little invasive procedure allows collection of milliliters of lymph per hour. So far no local tissue reaction or edema of peripheral tissues was observed. Exclusion of one lymphatic collector from drainage of leg tissue doesn’t affect lymphatic drainage due to many interconnections of peripheral lymphatics.
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© 2013 Springer Science+Business Media New York
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Olszewski, W.L. (2013). Collection of Human Limb Tissue Fluid and Lymph. In: Santambrogio, L. (eds) Immunology of the Lymphatic System. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3235-7_9
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DOI: https://doi.org/10.1007/978-1-4614-3235-7_9
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