Abstract
Straightforward electron microscopy has produced a wealth of information about subcellular biological structures. Up to a certain level of resolution, the interpretation of the images obtained is relatively straightforward. However, for a number of reasons which are discussed below, an objective interpretation of the fine details of biological structures requires relatively sophisticated techniques of image analysis.
This chapter was originally published in Journal of Microscopy, Vol. 100, Pt. 2, March 1974, pp. 143-152. Permission to republish it, with minor revisions, was kindly supplied by Blackwell Scientific Publications Ltd., Oxford and the Royal Microscopical Society.
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References
Amos, L. A., and Klug, A., 1972, Image filtering by computer, Proc. 5th Eur. Cong. Electron Microscopy, Manchester, pp. 580–581.
Crowther, R. A., and Arnos, L. A., 1971a, Harmonic analysis of electron microscope images with rotational symmetry, J. Mol. Biol. 60: 123–130.
Crowther, R. A., and Amos, L. A., 1971b, Three-dimensional image reconstructions of some small spherical viruses, Cold Spring Harbor Symp. Quant. Biol. 36: 489–494.
Crowther, R. A., and Franklin, R. M., 1972, The structure of the groups of nine hexons from adenovirus, J. Mol. Biol. 68: 181–184.
Crowther, R. A., Amos, L. A., and Klug, A., 1972, Three-dimensional image reconstruction using functional expansions, Proc. 5th Eur. Cong. Electron Microscopy, Manchester, pp. 593–597.
Crowther, R. A., DeRosier, D. J., and Klug, A., 1970, The reconstruction of a three-dimensional structure from projections and its application to electron microscopy, Proc. Roy. Soc. Lond. A317: 319–340.
DeRosier, D. J., and Klug, A., 1968, Reconstruction of three-dimensional structures from electron micrographs, Nature 217: 130–134.
Erickson, H. P., and Klug, A., 1971, Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographs, Phil. Trans. Roy. Soc. Lond. B261: 105–118.
Finch, J. T., and Klug, A., 1966, Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus, J. Mol. Biol. 15: 344–364.
Finch, J. T., and Klug, A., 1972, The helical surface lattice of bacterial flagella. In: The Generation of Subcellular Structures, First John Innes Symposium, Norwich (R. Markham and J. B. Bancroft, eds.), North-Holland Publ., Amsterdam, pp. 167–177.
Gilbert, P. F. C, 1972, Iterative methods for the three-dimensional reconstruction of an object from projections, J. Theor. Biol. 36: 105–117.
Gordon, R., Bender, R., and Herman, G. T., 1970, Algebraic reconstruction techniques (ART) for three-dimensional electron microscopy and X-ray photography, J. Theor. Biol. 29: 471–481.
Klug, A., and Berger, J. E., 1964, An optical method for the analysis of periodicities in electron micrographs, with some observations on the mechanism of negative staining, J. Mol. Biol. 10: 565–569.
Klug, A., and DeRosier, D. J., 1966, Optical filtering of electron micrographs: Reconstruction of one-sided images, Nature 212: 29–32.
Markham, R., Frey, S., and Hills, G. J., 1963, Methods for the enhancement of image detail and accentuation of structure in electron microscopy, Virology 20: 88–102.
Mellema, J. E., and Klug, A., 1972, Quaternary structure of gastropod haemocyanin, Nature 239: 146–150.
Yanagida, M., DeRosier, D. J., and Klug, A., 1972, The structure of tubular variants of the head of bacteriophage T4 (polyheads), J. Mol. Biol. 65: 489–499.
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© 1978 Plenum Press, New York
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Amos, L.A. (1978). Image Analysis of Macromolecular Structures. In: Batchelor, B.G. (eds) Pattern Recognition. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4154-3_11
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DOI: https://doi.org/10.1007/978-1-4613-4154-3_11
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