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Amplitude and Phase Contrast in X-Ray Microscopy

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Modern Microscopies

Abstract

When a material object is placed in the path of an electromagnetic wave, the latter undergoes a change in both its amplitude and its phase. In particular, calculations have been performed previously for two extremes(1) to obtain the contrast caused purely by the change in amplitude produced when an object is imaged by soft X rays (an amplitude object) or, alternatively, by the change in phase (a phase object) when imaged in a phase-contrast microscope with coherent illumination. These calculations show that phase contrast exceeded amplitude contrast. In fact, phase-contrast microradiography(2) and phase-contrast topography using x-ray interferometers(3) had already been suggested several years prior to the work reported here. To confirm these calculations, preliminary phase-contrast x-ray microscopy experiments were performed with the x-ray microscope at the BESSY electron storage ring.(4.5)

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References

  1. G. Schmahl and D. Rudolph, “Proposal for a phase-contrast x-ray microscope,” in: X-ray Microscopy: Instrumentation and Biological Applications (P. C. Cheng and G. J. Jan, eds.), pp. 231–238, Springer, Berlin (1987).

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  4. G. Schmahl, D. Rudolph, and P. Guttmann, “Phase-contrast x-ray microscopy—experiments at the BESSY storage ring,” in: X-Ray Microscopy II (D. Sayre, M. Howells, J. Kirz, and H. Rarback, eds), pp. 228–232, Springer, Berlin (1988).

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  5. D. Rudolph, B. Niemann, G. Schmahl, and O. Christ, “The Gottingen x-ray microscope and x- ray microscopy experiments at the BESSY storage ring,” in: X-Ray Microscopy (G. Schmahl and D. Rudolph, eds.), Springer Series in Optical Sciences, Vol. 43, pp. 192–202, Springer, Berlin (1984).

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This chapter is dedicated to Ulrich Bonse on the occasion of his 60th birthday.

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© 1990 Plenum Press, New York

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Rudolph, D., Schmahl, G., Niemann, B. (1990). Amplitude and Phase Contrast in X-Ray Microscopy. In: Duke, P.J., Michette, A.G. (eds) Modern Microscopies. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1467-7_4

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  • DOI: https://doi.org/10.1007/978-1-4613-1467-7_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8777-3

  • Online ISBN: 978-1-4613-1467-7

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