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Laser Fluorescent Microirradiation: A New Technique

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The Biomedical Laser

Abstract

The technique of fluorescence microscopy has been increasingly used for structural studies of biologic molecules (Konev, 1967; Udenfried, 1969; Duchesne, 1973). This technique is particularly valuable and sensitive for the investigation of biophysical and biochemical processes in single cells or organelles, even in the living state. Current trends in this field are, for example: (1) the biophysical study of the secondary structure of nucleic acids, (2) the biochemical study of enzymatic reactions that either produce fluorescent emission or occur on a fluorogenic substrate (Prenna et al., 1977; Kohen et al., 1978), and (3) the study of membrane properties related either to the potential-dependent release and uptake of fluorescent dyes or to the fluorescence recovery after photobleaching (Jacobson, 1979).

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Authors
  1. A. Andreoni
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  2. A. Longoni
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  3. C. A. Sacchi
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  4. O. Svelto
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Editor information

Editors and Affiliations

  1. Laser Laboratory, University of Cincinnati Medical Center, 231 Bethesda Avenue, 45267, Cincinnati, Ohio, USA

    Leon Goldman M.D. (Director) (Director)

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© 1981 Springer-Verlag New York Inc.

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Andreoni, A., Longoni, A., Sacchi, C.A., Svelto, O. (1981). Laser Fluorescent Microirradiation: A New Technique. In: Goldman, L. (eds) The Biomedical Laser. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5922-0_7

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  • DOI: https://doi.org/10.1007/978-1-4612-5922-0_7

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-5924-4

  • Online ISBN: 978-1-4612-5922-0

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