Abstract
The combination of freeze-fracture and immunocytochemical techniques permits to analyze the distribution of membrane components on both plasma and intracellular membranes (1–3). In particular the fracture-label approach, where cells are directly immunolabeled after freeze-fracture allows the exposition to the immunolabeling of the freeze-fractured inner nuclear membrane. Moreover, since during freeze-fracture, transmembrane proteins partition with the inner (protoplasmic) or outer (exoplasmic) leaflets of the freeze-fractured membrane, the fracture-label approach can also assess this partition. Therefore, we decided here to apply fracture-label technique to EBV producer lymphoblastoid cells in order to investigate the partition on fracture, distribution and extent of glycosylation of EBV envelope glycoproteins during the budding process.
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Torrisi, M.R. et al. (1989). Immunocytochemistry of EBV Envelope Glycoproteins on Freeze-Fractured Nuclear Membranes of Virus Producer Cells. In: Ablashi, D.V., Faggioni, A., Krueger, G.R.F., Pagano, J.S., Pearson, G.R. (eds) Epstein-Barr Virus and Human Disease • 1988. Experimental Biology and Medicine, vol 20. Humana Press. https://doi.org/10.1007/978-1-4612-4508-7_17
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DOI: https://doi.org/10.1007/978-1-4612-4508-7_17
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