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Nuclear Transfer in Mammals

  • Conference paper
In Vitro Fertilization and Embryo Transfer in Primates

Part of the book series: Serono Symposia, USA ((SERONOSYMP))

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Abstract

The transplantation of nuclei from one mammalian cell to another has provided a valuable tool for understanding developmental biology and for production of nearly identical animals useful in food production or research. The initial use of nuclear transfer was in amphibians 40 years ago when Briggs and King (1) reported that blastula stage nuclei transplanted to oocytes could direct development to the tadpole stage. They were testing the original hypothesis of Spemann (2), who suggested that cell totipotency and its loss with differentiation could be tested by transfer of nuclei from progressively advanced stages of development into enucleated oocytes. Continued study of cell totipotency in amphibia resulted in production of fertile frogs from blastula nuclei (3, 4), thereby demonstrating their totipotency. In rare cases even older embryonic and young larval cell nuclei from Xenopus directed formation of fertile frogs (5). However, nuclei from differentiated frog cells could not direct complete development (6). Completion of development failed in spite of the fact that transplanted nuclei were reprogrammed in nuclear composition by translocation of proteins from the oocyte cytoplasm (7, 8), and in a few cases individual genes turned off by differentiation were again expressed (9, 10). Nuclear transfer continues to be a useful tool in amphibia for the study of nuclear-cytoplasmic interactions, cell differentiation, and totipotency, as well as mechanisms regulating gene expression.

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Authors
  1. N. L. First
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  2. M. L. Leibfried-Rutledge
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Editor information

Editors and Affiliations

  1. Division of Reproductive Sciences, Oregon Regional Primate Research Center, 505 N.W. 185th Avenue, Beaverton, Oregon, 97006, USA

    Don P. Wolf Ph.D. , Richard L. Stouffer Ph.D.  & Robert M. Brenner Ph.D. ,  & 

  2. Department of Obstetrics/Gynecology, Oregon Health Sciences University, Portland, Oregon, USA

    Don P. Wolf Ph.D.

  3. Division of Reproductive Sciences, Oregon Health Sciences University, Portland, Oregon, USA

    Richard L. Stouffer Ph.D.

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© 1993 Springer-Verlag New York, Inc

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First, N.L., Leibfried-Rutledge, M.L. (1993). Nuclear Transfer in Mammals. In: Wolf, D.P., Stouffer, R.L., Brenner, R.M. (eds) In Vitro Fertilization and Embryo Transfer in Primates. Serono Symposia, USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2716-8_19

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  • DOI: https://doi.org/10.1007/978-1-4612-2716-8_19

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7640-1

  • Online ISBN: 978-1-4612-2716-8

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