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Characterization of Ion Channels in the Central Nervous System: Insights from Radioligand Binding, Autoradiography, and In Situ Hybridization Histochemistry

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Ion Channels and Ion Pumps

Part of the book series: Endocrinology and Metabolism ((EAM,volume 6))

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Abstract

Ion channels are molecules that form pores in the membrane to allow ion flow and several classes can be distinguished on the basis of their physiological properties.1 In turn, within each class of ion channel that exists in mammalian systems there is a large diversity of subtypes. I shall present some examples of scientific research, using the techniques of radioligand binding, in vitro receptor autoradiography, and more recently the technique of in situ hybridization histochemistry, which has increased our understanding of ion channel/receptor properties. In the process, I hope to illustrate the uses and advantages of these techniques, the resolution they provide, and their compatability with each other. It should be apparent, therefore, how the application of these techniques has allowed scientists to determine the existence of ion channel/receptor protein complexes and their subtypes, allosteric interactions between different types of ion channel-related receptor molecules, the cellular localization and ontogenic development of ion channel/receptors, and the effect of experimental and pathologic changes in neuronal function on various ion channels. Finally, future directions that research in these areas may take will be discussed along with the challenges that the ever-increasing diversity of ion channels present for neurochemists and neuropharmacologists.

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  1. Andrew L. Gundlach
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Editors and Affiliations

  1. 48323, 2104 Rhine Road, West Bloomfield, MI, USA

    Piero P. Foà M.D., Sc.D. (Professor Emeritus) (Professor Emeritus)

  2. Department of Physiology, Wayne State University, 48202, Detroit, MI, USA

    Piero P. Foà M.D., Sc.D. (Professor Emeritus) (Professor Emeritus)

  3. Section of Endocrinology,Department of Internal Medicine,School of Medicine, Wayne State University, 48202, Detroit, MI, USA

    Mary F. Walsh Ph.D. (Assistant Professor) (Assistant Professor)

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Gundlach, A.L. (1994). Characterization of Ion Channels in the Central Nervous System: Insights from Radioligand Binding, Autoradiography, and In Situ Hybridization Histochemistry. In: Foà, P.P., Walsh, M.F. (eds) Ion Channels and Ion Pumps. Endocrinology and Metabolism, vol 6. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2596-6_22

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