Abstract
In this article,a study of the dynamics of the immobilization of mesophilic anaerobic bacteria was conducted. Low-density polyethylene (LDPE) ground to fine particles was used as immobilization support in an inverse fluidized bed. After 135 d of immobilization, the biomass concentration attained was 375 µg protein/g dry plastic support. The specific rate for acetate consumption was 0. 008 µmol acetate/µg protein/h. The kinetic parameters of the bioparticles (K s = 1.067 mM and V max = 0. 00754 µmol acetate/µg protein/h) were estimated by the initial rate method at different acetate concentrations and were compared to the values obtained by a direct parameter estimation method from the dynamic assays for acetate consumption with an initial substrate concentration of 10 mM. The particles were prepared for scanning electron microscopy (SEM) to evaluate the biofilm qualitatively. The SEM studies showed that the surface and cavities of the support were colonized, and the bacterial structures found were bacilli similar to Methanothrix as the predominant microorganism and a few groups of Methanosarcina-like cocci. The K svalue found is between those reported for the predominant bacterial group immobilized.
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References
Henze, M. and Herremöes, P. (1983) Water Sci. Technol. 15, 1–101.
Gorris, L. G. M., Van Deursen, J. M. A., van der Drift, C., and Vogels, G. D. (1989) Biotechnol. Bioeng. 33, 687–693.
Binot, R. A., Bol, T., Naveau, H. P., and Nyns, E. J. (1983) Water Sci. Technol. 15, 103–115.
Iza, J. (1991), in Ananerobic Treatment Technology for Municipal and Industrial Wastewater, Switzenbaum, M. S., ed., International Association on Water Pollution Research and Control, Pergamon, Oxford, UK, pp. 109–132.
Guiot, S. (1992), Bioimmobilization in Anaerobic Reactors. Bioprocesos Anaerobios para el Tratamiento de Efluentes Industriales. UAM/IMP/ORSTOM. May 6–7, Mexico City.
Rubindamayugi, M. S. T., Op Den Camp, H. J. M., Lubberding, H. J., Gijzen, H. J., and Vogels, G. D. (1992) Water Sci. Technol. 25(1), 99–106.
Shapiro, M. and Switzenbaum, M. S. (1984) Biotechnol. Lett. 6(11), 729–734.
Verrier, D., Mortier, B., Dubourguier, H. C., and Albagnac, W. (1988), in Proceedings of the 5th International Symposium on Anaerobic Digestion. Hall, E. R. and Hobson, P. N., eds., Pergamon, Oxford, UK, pp. 61–69.
González, G., Ramírez, F., and Monroy, O. (1992) Biotechnol. Lett. 14(2), 149–154.
Meier-Schneiders, M., Busch, C., and Diekert, G. (1993) Appl. Microbiol. Biotechnol. 38, 667–673.
Zellner, G., Diekman, H., Austermann-Haun, U., and Seyfried, C. F. (1994) Biotechnol. Lett. 16(3), 611–620.
Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R., and Wolfe, R. S. (1979) Microbiol. Rev. 43(2), 260–296.
APHA-AWWA-WPCF. (1988) Standard Methods for the Examination of Water and Wastewater, 16th ed. Greenberg, A. E., Trussell, R. R., Clesceri, L. S., and Franson, A. A. H., eds., Port City Press, Baltimore, MD.
Sedmark, J. J. and Grossberg, S. E. (1977) Anal. Biochem. 79, 544–552.
Counotte, G. H. M. and Prins, R. A. (1979) Appl. Environ. Microbiol. 38(4), 758–760.
Pavlostathis, S. G. and Giraldo-Gómez, E. (1991), in Ananerobic Treatment Technology for Municipal and Industrial Wastewater, Switzenbaum, M. S., ed., International Association on Water Pollution Research and Control. Pergamon, Oxford, UK, pp. 35–60.
Guyot, J. P., Noyola, A., and Monroy, O. (1990) Biotechnol. Lett. 12, 155–160.
Kuba, T., Furumai, H., and Kusuda, T. (1990) Water Res. 24(11), 1365–1372.
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Meraz, M., González-Barrera, A., Alvarez-Ramirez, J., Monroy, O. (1996). Dynamics of the Immobilization of Anaerobic Meshopilic Bacteria on a Plastic Support. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_48
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DOI: https://doi.org/10.1007/978-1-4612-0223-3_48
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