Abstract
Following the excision of the tumor tissue and the conversion of the 3D tumor margins into a 2D plane, the tissue samples will have to be embedded and sectioned. There are two alternative commonly used techniques to prepare sections from the tumor samples which both produce sections suitable for a full diagnostics: paraffin sections or cryo-sections. In the following, both techniques will be discussed, and all required procedures for the preparation and the sectioning using either of them will be described and illustrated.
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Reference
Breuninger H, Schaumburg-Lever G. Control of excisional margins by conventional histopathological techniques in the treatment of skin tumours. An alternative to Mohs’ technique. J Pathol. 1988;154:167–71.
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Breuninger, H., Adam, P. (2013). Embedding and Sectioning. In: 3D Histology Evaluation of Dermatologic Surgery. Springer, London. https://doi.org/10.1007/978-1-4471-4438-0_3
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DOI: https://doi.org/10.1007/978-1-4471-4438-0_3
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