Abstract
Many analytical techniques involve the interaction of a sample with light (IR, visible and UV). While microwave radiation excites rotation of a molecule (ΔE ≈ 4kJ∕mol) and IR-light oscillatory movement of atoms around their position in a molecule (vibration, ΔE ≈ 40kJ∕mol), visible and UV light move electrons to higher energy levels (ΔE ≈ 400kJ∕mol). X-rays excite nucleons (ΔE ≈ 4000kJ∕mol).
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Buxbaum, E. (2011). Principles of Optical Spectroscopy. In: Biophysical Chemistry of Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7251-4_4
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DOI: https://doi.org/10.1007/978-1-4419-7251-4_4
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