Abstract
An important consideration in protein modification is the length of the spacer-arm. Fluorescence quantum yield of many labels, for example, is reduced if the label is too close to the protein. Steric hindrance may prevent the binding of proteins to affinity matrices if spacer length is insufficient. For special applications labile groups may be introduced into the spacer, for example disulphide-bonds (see p. 221). This allows the label to be removed again during an experiment.
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Buxbaum, E. (2011). General Technical Remarks. In: Biophysical Chemistry of Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7251-4_18
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DOI: https://doi.org/10.1007/978-1-4419-7251-4_18
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