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Genetics and Genomics of the Brassicaceae

Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 9))

Abstract

Metabolomics is developing toward an integral component of functional genomics approaches. The large structural diversity of plant metabolites requires different analytical techniques for broad metabolite analysis. In addition, new bioinformatics tools and databases are necessary for data analysis and storage. This chapter describes the resources available for comprehensive analysis of plant secondary metabolites focusing on Arabidopsis thaliana and Brassica species. In particular, a platform for non-targeted profiling of semi-polar plant metabolites based on liquid chromatography coupled to mass spectrometry is described.

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Abbreviations

APCI:

Atmospheric pressure chemical ionization

API:

Atmospheric pressure ionization

APPI:

Atmospheric pressure photoionization

CID:

Collision-induced dissociation

DGDG:

Digalactosyldiacylglycerol

ESI:

Electrospray ionization

FT-ICR:

Fourier-transform ion cyclotron resonance

GC:

Gas chromatography

LC:

Liquid chromatography

MS:

Mass spectrometry

MGDG:

Monogalactosyldiacylglycerol

NMR:

Nuclear magnetic resonance

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

ppb:

Parts per billion

ppm:

Parts per million

PS:

Phosphatidylserine

QTOF:

Quadrupole-time-of-flight

SQDG:

Sulfoquinovosyldiacylglycerol

UPLC:

Ultra-performance liquid chromatography

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  1. Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle/Saale, Germany

    Christoph Böttcher, Edda von Roepenack-Lahaye & Dierk Scheel

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  1. Christoph Böttcher
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  2. Edda von Roepenack-Lahaye
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Correspondence to Christoph Böttcher .

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  1. Kulturpflanzenforschung (IPK), Leibniz-Institut für Pflanzengenetik und, Corrensstr. 3, Gatersleben, 06466, Germany

    Renate Schmidt

  2. John Innes Institute, Norwich Research Park, Colney, Norwich, NR4 7UH, United Kingdom

    Ian Bancroft

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Böttcher, C., von Roepenack-Lahaye, E., Scheel, D. (2011). Resources for Metabolomics. In: Schmidt, R., Bancroft, I. (eds) Genetics and Genomics of the Brassicaceae. Plant Genetics and Genomics: Crops and Models, vol 9. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7118-0_17

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