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The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity

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Sperm Chromatin

Abstract

Here, an overview of the various clinical uses of processing of semen samples and cryopreservation is given. The effects of sperm processing on conventional semen parameters are discussed along with the ramifications of removing seminal plasma, oxidative stress and potential benefits of antioxidant addition in laboratory processing of testicular and ejaculated sperm. In the second part of the chapter, the effects of a second laboratory hazard, namely, cryopreservation, are discussed in terms of effects on conventional parameters of sperm structure and function and also on DNA fragmentation of testicular and ejaculated sperm. The greater vulnerability of sperm from infertile men is described, as well as the cryoinjury displayed by those with teratozoospermia. The mechanisms of cryoinjury are set out with special reference to oxidative damage and the process of repeated freezing and thawing. The efficacy of commercially available cryoprotectants is also discussed. Finally, novel freezing–thawing protocols such as freeze-drying and vitrification of sperm are explored.

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Authors and Affiliations

  1. Centre for Public Health, Queens University Belfast, Belfast, UK

    Dan Yu MD

  2. Centre for Public Health, Queens University Belfast, Institute of Clinical Sciences, Belfast, UK

    Luke Simon MSc, PhD

  3. Centre for Public Health, Queen’s University of Belfast, Room 208, Institute of Clinical Science, Belfast, Northern Ireland, UK

    Sheena E. M. Lewis PhD

Authors
  1. Dan Yu MD
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  2. Luke Simon MSc, PhD
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  3. Sheena E. M. Lewis PhD
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Corresponding author

Correspondence to Sheena E. M. Lewis PhD .

Editor information

Editors and Affiliations

  1. St. Mary's Hospital, Div. Urology, McGill University, Lacombe Avenue 3830, Montreal, H3T 1M5, Québec, Canada

    Armand Zini

  2. Lerner College of Medicine, Center for Reproductive Medicine, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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Yu, D., Simon, L., Lewis, S.E.M. (2011). The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity. In: Zini, A., Agarwal, A. (eds) Sperm Chromatin. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-6857-9_27

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  • DOI: https://doi.org/10.1007/978-1-4419-6857-9_27

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