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Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films

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Reviews in Fluorescence 2008

Part of the book series: Reviews in Fluorescence 2008 ((RFLU,volume 2008))

Abstract

Absorption and fluorescence spectroscopies with linearly polarized light are applied to study the anisotropic behavior of a fluorescence dye (rhodamine 6G, R6G) adsorbed in ordered clay (laponite, Lap) particles. Films elaborated by the spin-coating technique provide a parallel stacking of clay layers in the supported substrates. The posterior intercalation of the R6G molecules into the interlayer space of Lap films with a preferential orientation with respect to the normal to the clay layers gives rise to a macroscopic orientation of dye molecules into the 2D surfaces. Such an organization induces an anisotropic behavior with a photoresponse of dye/clay films to the plane of the polarized light. A mathematic procedure, based on the evolution of the fluorescence anisotropy with the twisting angle of the films with respect to the excitation light, is used to evaluate the preferential orientation of R6G molecules in Lap films. The fluorescence method can be extended to study the preferential orientation of fluorescent molecules adsorbed in any organized rigid 2D system.

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Notes

  1. 1.

    The dipole moment of the two excited states of the dimer would be the vectorial addition (algebraic addition and subtraction) of the individual dipole moment of the monomers. Such addition and subtraction will provide orthogonal dipole moments (see Fig. 10).

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Authors and Affiliations

  1. Departamento de Química Física, Universidad del País Vasco, Apartado 644, 48080, Bilbao, Spain

    F. López Arbeloa

  2. Departamento de Química Física, Universidad del País Vasco UPV/EHU, Apartado 644, 48080, Bilbao, Spain

    V. Martínez, T. Arbeloa & I. López Arbeloa

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  1. Institute of Fluorescence, University of Maryland, 701 E. Pratt St., Suites 3017-21, Baltimore, MD, 21202, USA

    Chris D. Geddes

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Arbeloa, F.L., Martínez, V., Arbeloa, T., Arbeloa, I.L. (2010). Fluorescence Anisotropy to Study the Preferential Orientation of Fluorophores in Ordered Bi-Dimensional Systems: Rhodamine 6G/Laponite Layered Films. In: Geddes, C.D. (eds) Reviews in Fluorescence 2008. Reviews in Fluorescence 2008, vol 2008. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1260-2_1

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