Embedding of Tissue for Frozen Section

Abstract

Embedding of tissue for frozen sectioning is a vital step in the preparation of tissue for intra-operative consultation. In many cases, this task needs to be performed with great precision or risk suboptimal interpretation and loss of precious tissue. Traditional forms of face up and face down embedding techniques are described along with potential sources of difficulty. The Precision Cryoembedding System, a series of highly precise face down embedding techniques developed by the author is discussed in detail. The system capitalizes on the physical property of tissue to adhere to freezing temperature metal. On face, on edge and on point embedding can be accomplished with ease using a number of approaches. Advantages include high precision, speed, minimal tissue wastage and reduction of freeze artifact. Techniques include face down embedding using freezing temperature steel well bars, paper embedding, cut off technique, plastering techniques and frozen block cryoembedding.