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In-Situ Observation of a Cell Growth Using Surface Infrared Spectroscopy

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 15))

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Abstract

In this study, we report on a novel method for in-situ observation of cell growth using surface infrared spectroscopy. Human breast cell line MCF-7 cells were cultured on small Si prisms and monitored using infrared absorption spectro­scopy in the geometry of multiple internal reflections (MIR-IRAS). Infrared spectra of cultured MCF-7 cells on Si prisms were collected for 300 min. The growth of MCF-7 cells was monitored by observing changes in the well-known “amide II” protein absorption band at 1,545 cm−1.

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Author information

Authors and Affiliations

  1. Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan

    Ko-ichiro Miyamoto, Takami Muto & Michio Niwano

  2. Alliance for Research on North Africa, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Parida Yamada & Hiroko Isoda

Authors
  1. Ko-ichiro Miyamoto
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  2. Takami Muto
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  3. Parida Yamada
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  4. Michio Niwano
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  5. Hiroko Isoda
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Editor information

Editors and Affiliations

  1. Dept. Genetic Resources Technology, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

    Sanetaka Shirahata

  2. Dept. Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan

    Koji Ikura

  3. Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan

    Masaya Nagao

  4. Dept. Applied Biology, Kyoto Institute of Technology, Matsugasaki, Kyoto, 606-8585, Japan

    Akira Ichikawa

  5. Fac. Agriculture, Kyushu University, Hakozaki, Fukuoka, 812-8581, Japan

    Kiichiro Teruya

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© 2008 Springer Science+Business Media B.V.

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Miyamoto, Ki., Muto, T., Yamada, P., Niwano, M., Isoda, H. (2008). In-Situ Observation of a Cell Growth Using Surface Infrared Spectroscopy. In: Shirahata, S., Ikura, K., Nagao, M., Ichikawa, A., Teruya, K. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9646-4_9

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