Abstract
The effect of serum on cell growth and virus production in both basal and serum free media was examined in Human Embryonic Kidney (HEK) 293. A 40-fold decrease in virus titre was observed in Dulbecco’s Modified Eagles Medium (DMEM) cultures supplemented with 10% FBS when compared to 1% FBS. However, a 90-fold increase in virus titre was observed in DMEM supplemented with 1% FBS as compared with EX-CELL™ 293 serum free culture. A similar effect was also seen for the efficiency of gene transfer as indicated by the expression level of GFP. The results demonstrated that although serum at higher concentrations has a negative and dose-dependent effect on titre its supplementation in small quantity is essential for virus productivity. The effect of Insulin-like growth factors on proliferation of HEK 293 cells as well as on infection and specific productivity was also studied in serum free media. It was found that LONG™ R3 IGF-1, an analogue of IGF-1 promotes cell proliferation and viability more effectively than Insulin and IGF-1. Similarly, cultures supplemented with LONG™ R3 IGF-1 showed relatively higher virus productivity in comparison to other supplemented cultures but virus productivity was found to be significantly (p < 0.05) higher when there were no growth factors at all.
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References
Graham, F. L. et al. (1977) Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5. Journal of General Virology 36 (1): 59–72.
Yandell, C. et al. (2004) An Anolgue of IGF-1. A Potent Substitute for Insulin in Serum-Free Manufacture of Biologics by CHO Cells. Bioprocess International 56–64.
Sunstrom, N. A., Baig, M., Cheng, L., Sugyiono, D. P., and Gray, P. (1998) Recombinant Insulin-Like Growth Factor-I (IGF-1) Production in Super-CHO Results in the Expression of IGF-1 receptor and IGF Binding Protein 3. Cytotechnolgy 28: 91–99.
Francis, G. L., Ross, M., Ballard, F. J., Milner, S. J., Senn, C., McNeil, K. A., Wallace, J. C., King, R. and Wells, J. R. (1992) Novel Recombinant Fusion Protein Analogues of Insulin-Like Growth Factor (IGF)-I Indicate the Relative Importance of IGF-Binding Protein and Receptor Binding for Enhanced Biological Potency. Journal of Molecular Endocrinology 8 (3): 213–223.
Gueret, V., Negrete-Virgen, J. A., Lyddiatt, A. and Al-Rubeai, M. (2002) Rapid Titration of Adenoviral Infectivity by Flow Cytometry in Batch Culture of Infected HEK 293 Cells. Cytotechnology 38: 87–97.
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© 2008 Springer Science+Business Media B.V.
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Buckler, A., Al-Rubeai, M. (2008). Effects of Serum and Growth Factors on HEK 293 Proliferation and Adenovirus Productivity. In: Shirahata, S., Ikura, K., Nagao, M., Ichikawa, A., Teruya, K. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9646-4_3
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DOI: https://doi.org/10.1007/978-1-4020-9646-4_3
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