Abstract
Rice blast disease, caused by the fungus Magnaporthe oryzae, is a leading constraint to rice production and is a serious threat to food security worldwide. To elucidate the function of effector proteins from M. oryzae in pathogenesis and interaction with the host, we have performed RL-SAGE and MPSS approaches to study the gene expression profiles of M. oryzae during the interaction. The RL-SAGE and MPSS analyses identified 3,441 and 3,004 annotated M. oryzae genes from blast infected rice leaf tissues, respectively. Among them, 217 genes encoding putative secreted proteins, which may play important roles as effectors, were identified. We developed a highly efficient transient protoplast system for gene functional analysis in rice. We present here the detailed procedure of the rice protoplast transient expression system and show the examples of using the system for functional analysis of putative effectors from M. oryzae. The combination of RL-SAGE/MPSS genomic profiling approaches with a protoplast functional assay system has provided an efficient approach for large-scale isolation and analysis of effectors from M. oryzae.
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Chen, S. et al. (2009). Isolation and Functional Analysis of Putative Effectors from Magnaporthe oryzae Using Integrated Genomic Approaches. In: Wang, GL., Valent, B. (eds) Advances in Genetics, Genomics and Control of Rice Blast Disease. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9500-9_10
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DOI: https://doi.org/10.1007/978-1-4020-9500-9_10
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