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Propagation of Sweetpotatoes, In Situ Germplasm Conservation and Conservation by Tissue Culture

  • Chapter
The Sweetpotato

Propagation of sweetpotatoes is largely by vegetative propagation, which plays a major role in sweetpotato agriculture, improvement, conservation and diversity. Several topics involving propagation will be covered, including in situ germplasm conservation, virus cleaning by tissue culture, mass propagation by tissue culture, germplasm conservation by tissue culture, and the use of tissue culture for the international transport of sweetpotato germplasm.

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References

  • Alconero, R., Santiago, A.G., Morales, F. and Rodriguez, F. 1975. Meristem tip culture and virus indexing of sweet potatoes. Phytopathology 65 :769–773.

    Article  Google Scholar 

  • Al-Mazrooei, S., Bhatti, M.H., Henshaw, G.G., Taylor, N.J. and Blakesley, D. 1997. Optimisation of somatic embryogenesis in fourteen cultivars of sweet potato (Ipomoea batatas (L.) Lam.). Plant Cell Rep. 16 :710–714.

    Article  CAS  Google Scholar 

  • Bhatti, M.H., Percival, T., Davey, C.D.M., Henshaw, G.G. and Blakesley, D. 1997. Cryopreservation of embryogenic tissue of a range of genotypes of sweet potato (Ipomoea batatas (L) Lam.) using an encapsulation protocol. Plant Cell Rep. 16 :802–806.

    Article  CAS  Google Scholar 

  • Bieniek, M.E., Harrell, R.C. and Cantliffe, D.J. 1995. Enhancement of somatic embryogenesis of Ipomoea batatas in solid cultures and production of mature somatic embryos in liquid cultures for application to a bioreactor production system. Plant Cell Tissue Organ Cult. 41 :1–8.

    Article  Google Scholar 

  • Boncodin, R.M. 2002. Root crop genetic resources R&D in the Philippines: a review of UPWARD experiences. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 159–167.

    Google Scholar 

  • CIP. 2007a. Global strategy for ex-situ conservation of sweetpotato genetic resources. Sweetpotato Genetic Resources Conservation – CIP Genebank November 2007. http:// www.croptrust.org/documents/web/SweetPotato-Strategy-FINAL-12Dec07.pdf (link checked 7 April 2008).

  • CIP. 2007b. Workshop on “Clonal crops germplasm management” Lima, Peru, Nov 12–16, 2007. http://research.cip.cgiar.org/confluence/download/attachments/13021/Sweetpotato$+$and $+$ARTCs_GPG2a.pdf (link checked 11 March 2008).

  • CIP. 2008. http://research.cip.cgiar.org/confluence/display/SMTA/Important+information (link checked 11 March 2008).

  • Dangler, J.M., Scheuerman, R.W., Campbell, R.N. and Clark, C.A. 1994. Meristem-tip culture and California’s clean foundation sweetpotato program. HortTechnology 4 :227–228.

    Google Scholar 

  • DeWald, S.G. and Cantliffe, D.J. 1988. Histology of callus initiation and somatic embryogenesis from sweet potato (Ipomoea batatas Poir.) shoot apices. HortScience 23 :812.

    Google Scholar 

  • Frison, E.A. and Ng, S.Y. 1981. Elimination of sweet potato virus disease agents by meristem tip culture. Trop. Pest Manag. 27 :452–454.

    Article  Google Scholar 

  • Fuglie, K.O., Mahalaya, S. and Suri, F. 2002. The economics of sweetpotato genetic resource conservation and varietal improvement in Asia. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 79–122.

    Google Scholar 

  • Gamborg, O., Miller, R. and Ojima, K. 1968. Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50 :151–158.

    Article  PubMed  CAS  Google Scholar 

  • Golmirzaie, A. and Toledo, J. 1998. In vitro conservation of potato and sweet potato germplasm. CIP Program report 1997–8, pp. 351–356. http://www.cipotato.org/publications/program_ reports/97_98/41invitr.pdf (link checked 7 April 2008).

  • Guo, X.D., Zhou, M.D., and Wang, Y. 2001. In vitro conservation of sweetpotato germplasm. In: Rao V.R. and Hermann M. (eds), Conservation and Utilization of Sweetpotato Genetic Diversity in Asia, pp. 16–24.

    Google Scholar 

  • Heo, J.W., Wilson, S.B. and Kozai, T. 2001. A forced ventilation micropropagation system for photoautotrophic production of sweetpotato plug plantlets in a scaled-up culture vessel: I. Growth and uniformity. HortTechnology 11 :90–94.

    Google Scholar 

  • Henderson, J.H.M., Phills, B.R. and Whatley B.T. 1984. Sweet Potato. In: Sharp, W.R., Evans, D.A., Ammirato, P.V., Yamada, Y. (eds), Handbook of Plant Cell Culture, Volume 2. Crop species, Macmillan, New York, pp. 302–326.

    Google Scholar 

  • Hirai, D. and Sakai A. 2003. Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection. Plant Cell Rep. 21 :961–96.

    Article  PubMed  CAS  Google Scholar 

  • Huett, D.O. 1982. Evaluation of sources of propagating-material for sweet-potato production. Sci. Hortic. 16 :1–7.

    Article  Google Scholar 

  • Islam, A.F.M.S., Zobayed, S.M.A., Hossain, K.G. and Kozai, T. 2004. Photoautotrophic growth of sweet potato plantlets in vitro as affected by root supporting materials, CO2 concentration, and photosynthetic photon flux. Trop. Agric. 81 :80–86.

    Google Scholar 

  • Jarret, R.L. 1989. A repository for sweetpotato germplasm. HortScience 24 :885–8.

    Google Scholar 

  • Jarret, R.L. and Gawel, N. 1991a. Abscisic acid-induced growth inhibition of sweet potato (Ipomoea batatas L.) in vitro. Plant Cell, Tissue Organ Cult. 24 :13–18.

    Article  CAS  Google Scholar 

  • Jarret, R.L. and Gawel, N. 1991b. Chemical and environmental growth regulation of sweetpotato (Ipomoea batatas (L.) Lam.) in vitro. Plant Cell, Tissue Organ Cult. 25 :153–159.

    CAS  Google Scholar 

  • Jarret R.L. and Florkowski W.J. 1990. In vitro active vs. field genebank maintenance of sweet potato germplasm: major costs and considerations. HortScience 25 :141–146.

    Google Scholar 

  • Kuo, C.G., Shen, B.J., Shen, M.J., Green, S.K. and Lee, D.R. 1985. Virus-free sweet potato storage roots derived from meristem-tips and leaf-cuttings. Sci. Hortic. 26 :231–240.

    Article  Google Scholar 

  • Kuo, C.G. 1991. Conservation and distribution of sweet potato germplasm. In: Dodds J.H. (ed), In Vitro Methods for Conservation of Plant Genetic Resources. Chapman and Hall, London. Pp 123–149.

    Google Scholar 

  • Liao, C.H. and Chung, M.L. 1979. Shoot tip culture and virus indexing in sweet potato. J. Agric. Res. China 28: 139–144.

    Google Scholar 

  • Liu, Q.C., Zhai, H., Wang, Y. and Zhang, D.P. 2001. Efficient plant regeneration from embryogenic suspension cultures of sweetpotato. In Vitro Cell. Develop. Biol. - Plant 37 :564–567.

    Article  Google Scholar 

  • Love, S.L., Rhodes, B.B. and Moyer, J.W. 1987. Meristem-tip culture and virus indexing of sweet potatoes. Practical manuals for handling crop germplasm in vitro 1. International Board for Plant Genetic Resources. IBPGR Headquarters, Rome, 1987. http://www. bioversityinternational.org/publications/Web_version/117 (link checked 11 March 2008).

    Google Scholar 

  • Matsumoto, T., Sakai, A., Takahashi, C., and Yamada, K. 1995. Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by encapsulation-vitrification method. Cryo-Letters 16 :189–196.

    Google Scholar 

  • Marco, S. and Walkey, D.G.A. 1992. Rapid propagation of sweet potato (Ipomoea batatas) by meristem tip culture in relation to virus eradication. Phytopathol. Mediterr. 31 :185–187.

    Google Scholar 

  • Mori, K. 1971. Production of virus-free plant by means of meristem culture. J. Agric. Res. Quart. 6 :1–7.

    Google Scholar 

  • Moyer, J.W. and Collins W.W. 1983. “Scarlet” sweet potato. HortScience 18 :111–112.

    Google Scholar 

  • Moyer, J.W., Jackson, G.V.H. and Frison, E.A. (eds), 1989. FAO/IBPGR Technical Guidelines for the Safe Movement of Sweet Potato Germplasm. Food and Agriculture Organization of the United Nations, Rome/International Board for Plant Genetic Resources, Rome. http://www. bioversityinternational.org/publications/Pdf/503.pdf (link checked 11 March 2008).

  • Murashige T. and Skoog, F. 1962. A revised medium for rapid growth and bio-assay with tobacco tissue cultures. Physiol. Plant. 15 :473–497.

    Article  CAS  Google Scholar 

  • Naskar, S.K., Amma, C.S.E. and Mukherjee, A. 2002. Present status of sweetpotato genetic resources in India. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 33–34.

    Google Scholar 

  • Nakatani, M. 2002. From ex situ to in situ: Japan’s domestic and international experiences. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 166–168.

    Google Scholar 

  • NCGRP, 2007. http://www.ars.usda.gov/Main/docs.htm?docid=13859 (checked 11 March 2008).

  • Nielsen, L.W. 1960. Elimination of internal cork virus by culturing apical meristems of infected sweetpotatoes. Phytopathology 50 :840–841.

    Google Scholar 

  • Padmanabhan, K, Cantliffe, D.J., Harrell, R.C. and Harrison, J. 1998. Computer vision analysis of somatic embryos of sweet potato [Ipomoea batatas (L.) Lam.] for assessing their ability to convert to plants. Plant Cell Rep. 17 :681–684.

    Article  CAS  Google Scholar 

  • Paek, K.Y., Chakrabarty, D. and Hahn, E.J. 2001. Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell. Develop. Biol. - Plant 37 :149–157.

    Article  CAS  Google Scholar 

  • Panta, A., Espinoza, C. and Roca W. 2007. Health status testing and virus elimination in sweetpotato.http://research.cip.cgiar.org/confluence/download/attachments/13542/4.+SP+ Virus+elimination.pdf?version=1 (link checked 11 March 2008).

  • Reed, B.M. 2008. Plant Cryopreservation: A Practical Guide. (editor) Springer.

    Google Scholar 

  • Reed, B.M., Engelmann, F., Dulloo, M.E. and Engels, J.M.M. 2004. Technical guidelines for the management of field and in vitro germplasm collections. IPGRI Handbooks for Genebanks No. 7. International Plant Genetic Resources Institute, Rome, Italy. http://www.bioversityinternational.org/Publications/Pdf/1016.pdf (link checked 7 April 2008).

    Google Scholar 

  • Rao, V.R. and Campilan D. 2002. Exploring the complementarities of in situ and ex situ conservation strategies for asian sweetpotato genetic resources. Proceedings of 3rd International Workshop Asian Network for Sweetpotato Genetic Resources (ANSWER) held in Denpasar, Bali, Indonesia on 2–4 October, 2001. International Plant Genetic Resources Institute Regional Office for Asia, the Pacific and Oceania (IPGRI-APO), Serdang, Malaysia (editors). (pdf available from http://www.bioversityinternational.org/index.asp).

  • Rao, V.R. and Hermann, M. 2001. Conservation and utilization of sweetpotato genetic diversity in Asia. Proceedings of the Asian Network for Sweetpotato Genetic Resources Workshop, 3–5 November 1999, Bogor, Indonesia. IPGRI-APO, Serdang, Malaysia (editors). http://www.bioversityinternational.org/Publications/Pdf/739.pdf (link checked 7 April 2008).

  • Rao, V.R. and Sthapit, B. 2002. In situ conservation – a component of complementary conservation strategy. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 123–135.

    Google Scholar 

  • Schultheis, J.R., Cantliffe, D.J. and Chee, R.P. 1990. Optimizing sweet potato (Ipomoea batatas (L.) Lam.) root and plantlet formation by selection of proper embryo developmental stage and size, and gel type for fluidized sowing. Plant Cell Rep. 9 :356–359.

    Article  Google Scholar 

  • Sthapit, B., Rao, V.R. and Jarvis, D. 2002. Conceptual basis of In situ conservation of sweetpotato agrobiodiversity. In: Rao, V.R. and Campilan D. (eds), Exploring the Complementarities of in Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, IPGRI 2002. pp. 136–141.

    Google Scholar 

  • Torres, A.C., Ze, N.M. and Cantliffe, D.J. 2001. Abscisic acid and osmotic induction of synchronous somatic embryo development of sweet potato. In Vitro Cell. Develop. Biol - Plant 37 :262–267.

    Article  CAS  Google Scholar 

  • Towill, L.E. 2005. Germplasm preservation. In: Trigiano, R.N. and Gray, D.J. (eds), Plant Development and Biotechnology. CRC Press, Boca Raton, FL, pp. 277–284.

    Google Scholar 

  • Villordon, A.Q. and LaBonte, D.R. 1996. Genetic variation among sweetpotatoes propagated through nodal and adventitious sprouts. J. Am. Soc. Hort. Sci. 121 :170–174.

    Google Scholar 

  • Volk, G.M. and Walters, C. 2003. Preservation of genetic resources in the National Plant Germplasm Clonal collections. Plant Breeding Rev. 23 :291–344.

    Google Scholar 

  • Yaku, A. and Widyastuti, C.A. 2002. In situ conservation of sweetpotato: an Irian Jaya experience. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 142–149.

    Google Scholar 

  • Yen, D.E. 1974. The Sweetpotato and Oceania: An Essay in Ethobotany.Bulletin 236, Bishop Museum Press, Honolulu, Hawaii, pp. 1–389.

    Google Scholar 

  • Widyastuti, C.A., Prain, G. and Yaku, A. 2002. Dani women’s knowledge on and its contribution to maintenance of sweetpotato diversity in Baliem Valley. In: Rao, V.R. and Campilan, D (eds), Exploring the Complementarities of In Situ and Ex Situ Conservation Strategies for Asian Sweetpotato Genetic Resources, pp. 150–158.

    Google Scholar 

  • Zheng, Qi., Dessai, A.P. and Prakash, C.S. 1996. Rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. Plant Cell Rep. 15 :381–385.

    Article  CAS  Google Scholar 

  • Zobayed, S. M.A., Afreen, F., Kubota, C. and Kozai, T. 2000. Water control and survival of Ipomoea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilation. Ann. Bot. 86 :603–610.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Pathology and Weed Science, ARO Volcani Center, POB 6, Bet Dagan 50250, Israel

    V. Gaba & Sima Singer

Authors
  1. V. Gaba
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  2. Sima Singer
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Editors and Affiliations

  1. Dept. Virology, Agricultural Research Organization, Bet Degan, Israel

    Gad Loebenstein

  2. Sahrdaya College of Engineering, Teekay Villa, P.O. Karoor Via. Thazhekkad-680697, Trichur Dist.,, Kerala, India

    George Thottappilly

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Gaba, V., Singer, S. (2009). Propagation of Sweetpotatoes, In Situ Germplasm Conservation and Conservation by Tissue Culture. In: Loebenstein, G., Thottappilly, G. (eds) The Sweetpotato. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9475-0_6

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