Abstract
This report summarises practical aspects to measuring cell motility in culture. The methods described here were discussed at a 1-day European Tissue Culture Society (ETCS-UK) workshop organised by John Masters and Gareth E Jones that was held at University College London on 19th April 2007.
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Dahlmann-Noor AH, Martin-Martin B, Eastwood M, Khaw PT, Bailly M. Dynamic protrusive cell behaviour generates force and drives early matrix contraction by fibroblasts. Exp Cell Res. 2007;313:4158–69.
Denker SP, Barber DL. Cell migration requires both ion translocation and cytoskeletal anchoring by the Na-H exchanger NHE1. J Cell Biol 2002;159:1087–96.
Hodivala-Dilke KM, DiPersio CM, Kreidberg JA, Hynes RO. Novel roles for alpha3beta1 integrin as a regulator of cytoskeletal assembly and as a trans-dominant inhibitor of integrin receptor function in mouse keratinocytes. J Cell Biol 1998;142:1357–69.
Hodivala-Dilke KM, Reynolds AR, Reynolds LE. Integrins in angiogenesis: multitalented molecules in a balancing act. Cell Tissue Res 2003;314:131–44.
Hooper S, Marshall JF, Sahai E. Tumor cell migration in three dimensions. Methods Enzymol 2006;406:625–43.
Ivetic A, Florey O, Deka J, Haskard DO, Ager A, Ridley AJ. Mutagenesis of the ezrin–radixin–moesin binding domain of l-selectin tail affects shedding, microvillar positioning, and leukocyte tethering. J Biol Chem 2004;279:33263–72.
Jones GE, Ridley AJ, Zicha D. Rho GTPases and cell migration: measurement of macrophage chemotaxis. Methods Enzymol 2000;325:449–62.
Kellie S, Craggs G, Bird IN, Jones GE. The tyrosine phosphatase DEP-1 induces cytoskeletal rearrangements, aberrant cell-substratum interactions and a reduction in cell proliferation. J Cell Sci 2004;117:609–18.
Millán J, Williams L, Ridley AJ. An in vitro model to study the role of endothelial rho GTPases during leukocyte transendothelial migration. Methods Enzymol 2006;406:643–55.
Ramsay AG, Keppler MD, Jazayeri M, Thomas GJ, Parsons M, Violette S, et al. HS1-associated protein X-1 regulates carcinoma cell migration and invasion via clathrin-mediated endocytosis of integrin alphavbeta6. Cancer Res 2007;67:5275–84.
Redd MJ, Kelly G, Dunn G, Way M, Martin P. Imaging macrophage chemotaxis in vivo: studies of microtubule function in zebrafish wound inflammation. Cell Motil Cytoskeleton 2006;63:415–22.
Rhee S, Jiang H, Ho CH, Grinnell F. Microtubule function in fibroblast spreading is modulated according to the tension state of cell–matrix interactions. Proc Natl Acad Sci U S A 2007;104:5425–30.
Turner LJ, Hall A. Plexin-induced collapse assay in COS cells. Methods Enzymol 2006;406:665–76.
Wells CM, Ahmed T, Masters JR, Jones GE. Rho family GTPases are activated during HGF-stimulated prostate cancer-cell scattering. Cell Motil Cytoskeleton 2005;62:180–94.
Woolner S, Jacinto A, Martin P. The small GTPase Rac plays multiple roles in epithelial sheet fusion–dynamic studies of Drosophila dorsal closure. Dev Biol 2005;282:163–73.
Further reading
Blow N. Cell migration: our protruding knowledge. Nature Methods 2007;4:589–94.
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Hague, A., Jones, G.E. (2007). Cell motility assays. In: Hunt, C. (eds) Proceedings of the VIIIth Conference of the International Society for Trace Element Research in Humans (ISTERH), the IXth Conference of the Nordic Trace Element Society (NTES), and the VIth Conference of the Hellenic Trace Element Society (HTES), 2007. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9056-1_8
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DOI: https://doi.org/10.1007/978-1-4020-9056-1_8
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