Abstract
The doubled haploid (DH) technique is routinely applied in winter rapeseed breeding programmes for the generation of completely homozygous lines. Although the method is quite old and perfected, there are still some problems that prevent a universal application of the technique in winter rapeseed breeding. Key problems remain the insufficient diploidisation rate, the low seed yield of primary haploids and the time-consuming and inadequate plantlet regeneration from the embryos. This contribution summarizes current applications and approaches to tackle above problems. Methods of early in vitro selection of microspore derived embryo genotypes are presented that appear promising for a further optimisation of the DH technology in rapeseed.
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Möllers, C., Iqbal, M.C.M. (2009). Doubled Haploids in Breeding Winter Oilseed Rape. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_13
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DOI: https://doi.org/10.1007/978-1-4020-8854-4_13
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