There are many techniques available for the quantification of plant-associated biological nitrogen fixation (BNF) and they can be divided roughly into those that are applicable in the field and those that are not. This short review will mainly consider the former and we cite key references that should be consulted for further details. The techniques that are rarely applicable in the field include the acetylene-reduction assay, exposure to 15N2-enriched atmospheres and the total N-balance method.
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Boddey, R.M., Jantalia, C.P., Zotarelli, L., Okito, A., Alves, B.J.R., Urquiaga, S. (2008). Techniques for the Quantification of Plant-Associated Biological Nitrogen Fixation. In: Dakora, F.D., Chimphango, S.B.M., Valentine, A.J., Elmerich, C., Newton, W.E. (eds) Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture. Current Plant Science and Biotechnology in Agriculture, vol 42. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8252-8_12
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DOI: https://doi.org/10.1007/978-1-4020-8252-8_12
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