Abstract
During the past two decades, significant advancements have been made in the field of molecular ecology and epidemiology, resulting in accumulation of large amount of genomic data. Application of molecular techniques has been demonstrated to be essential for rapid, reliable and reproducible results for detection, identification and differentiation of microbial plant pathogens. The specificity of reactions of molecular techniques has been shown to be a clear advantage over conventional methods. Establishment of the identity of the microbial pathogen is the basic requirement of epidemiological investigations. Equally important is the possibility of quantification of pathogen(s) present in plants, air, soil and water by molecular techniques adding credibility to the conclusions arrived at. The effects of host plant, pathogen and environment on the incidence and spread of the diseases have been assessed by using molecular methods. The interactions between the pathogens, particularly viruses and their vectors has been studied in detail in some pathosystems. Molecular methods have opened up the possibility of having an insight into the disease development from different sources of infection which may or may not exhibit symptoms of infection.
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(2008). Molecular Ecology and Epidemiology. In: Molecular Biology in Plant Pathogenesis and Disease Management. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8245-0_3
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