In principle, PCR generates large quantities of DNA from a minute amount of nucleic acid starting material using a methodology similar to (but much simpler than) that seen in living cells. For living cells, in vivo DNA synthesis is dependent upon a well defined but complex set of enzymes and co-factors, which have evolved to act in a concerted fashion during the synthetic phase (S-phase) of the cell cycle. In comparison, PCR facilitates in vitro DNA synthesis in a much simpler fashion, making use of a smaller set of defined ingredients and reaction conditions involving relatively high temperatures. The range of factors contributing to successful PCR amplification is reviewed below.
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Keywords
- Exonuclease Activity
- Deoxynucleotide Triphosphate
- Exonuclease Domain
- Concerted Fashion
- Single Strand Binding Protein
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(2008). A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification. In: Principles and Technical Aspects of PCR Amplification. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6241-4_2
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