Enhanced solar ultraviolet radiation (UVR) due to stratospheric ozone depletion is a major stress factor for many phototrophic organisms in aquatic and terrestrial ecosystems (Franklin and Forster, 1997). UVR includes the wavelengths below those visible to the human eye. According to the CIE (Commission Internationale de l’Eclairage), the spectral range is divided into three wavebands: 315–400 nm UVA, 280–315 nm UVB and 190–280 nm UVC. UVA is not attenuated by ozone, and hence its fluence rate will be unaffected by any ozone layer reduction reaching aquatic and terrestrial organisms. Increases in UVB have been particularly reported in Antarctica (McKenzie et al., 2003) and the adjacent geographic regions (southern parts of South America and Australia) (Buchdahl, 2002; Deschamps et al., 2004), as well as in more recent years in the Arctic region (Knudsen et al., 2005). UVB exposure is potentially harmful to all living organisms, but especially to photosynthetic organisms due to their requirement for light. UVB represents less than 1% of the total solar radiation reaching the earth’s surface, because it is absorbed partly by the ozone layer. It is particularly this waveband, which is influenced by changing stratospheric ozone concentrations caused by anthropogenic emissions of greenhouse gases, such as chlorinated fluorocarbons (Fraser et al., 1992).
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References
Adhikary, S.P. and Sahu, J.K. (2000). Survival strategies of cyanobacteria occurring as crust in the rice fields under drought conditions. Ind. J. Microbiol. 40: 53-56.
Bebout, B.M. and Garcia-Pichel, F. (1995). UV-Binduced vertical migrations of cyanobacteria in a microbial mat. Appl. Environ. Microbiol. 61:4215-4222.
Böhm, G.A., Pfleiderer, W., Böger, P. and Scherer, S. (1995). Structure of a novel oligosaccharide mycosporine amino acid ultraviolet A/B sunscreen pigment from the terrestrial cyanobacterium Nostoc commune. J. biol. Chem. 270: 8536-8539.
Brenowitz, S. and Castenholz, R.W. (1997). Long-term effects of UV and visible irradiance on natu-ral populations of scytonemin-containing cyanobacterium (Calothrix sp.). FEMS Microbiol. Ecol. 24: 343-352.
Büdel, B., Karsten, U. and Garcia-Pichel, F. (1997). Ultraviolet-absorbing scytonemin and mycosporine like amino acid derivatives inexposed rock inhabiting cyanobacterial lichens. Oecologia 112: 165-172.
Büdel, B. (1999). Ecology and diversity of rock-inhabiting cyanobacteria in tropical regions. Eur. J. Phycol. 34: 361-370.
Buchdahl, J. (2002). Ozone depletion. Available at http://www.ace.mmu.ac.uk/Resources/Fact_Sheets/ Key_Stage_4/Ozone_Depletion/pdf/Ozone_Depletion. Accesed on 22 May 2007.
Burton, G.W. and Ingold, K.U. (1984). Carotene: an unusual type of lipid antioxidant. Science 224: 569-573.
Cadenas, E. (1989). Biochemistry of oxygen toxicity. Annu. Rev. Biochem. 58: 79-110.
Cadoret, J.C., Rousseau, B., Perewoska, I., Sicora, C., Cheregi, O., Vass, I. and Houmard, J. (2005). Cyclic nucleotides, the photosynthetic apparatus and response to a UV-B stress in the cyanobac-terium Synechocystis sp. PCC 6803. J. Biol. Chem. 280: 33935-33944.
Campbell, D., Eriksson, M.J., Öquist, G., Gustafsson, P. and Clarke, A.K. (1998). The cyanobac-terium Synechococcus resists UV-B by exchanging photosystem II reaction-center D1 proteins. Proc. Natl. Acad. Sci. U.S.A. 95: 364-369.
Canfield, D.E., Sorensen, K.B. and Oren, A. (2004). Biogeochemistry of a gypsum-encrusted micro-bial ecosystem. Geobiology 2: 133-150.
Castenholz, R.W. (1972). Low temperature acclimation and survival in thermophilic Oscillatoria tere-briformis. In: T.V. Desikachary (ed.) Taxonomy and Biology of Blue-Green Algae. University of Madras, India, pp. 406-418.
Castenholz, R.W. (1997). Multiple strategies for UV tolerance in cyanobacteria. Spectrum 10: 10-16.
Castenholz, R.W. and Garcia-Pichel, F. (2000). Cyanobacterial responses to UV-radiation, In: B.A. Whitton and M. Potts (eds.) The Ecology of Cyanobacteria. Kluwer Academic Publishers, Netherlands, pp. 591-611.
Christensen, B.E., Kjosbakken, J. and Smidsrod, O. (1985). Partial chemical and physical characteri-zation of two extracellular polysaccharides produced by marine periphytic Pseudomonas sp. Strain NCMB 2021. Appl. Environ. Microbiol. 50: 837-845.
Cockell, C.S. and Knowland, J. (1999). Ultraviolet radiation screening compounds. Biol. Rev. 74: 311-345.
Deschamps, L., Roff, G., Fraser, P., Klekociuk, A. and Grainger, S. (2004). Ozone and UV September 2004. BMRC Res. Lett. 1: 1-4.
Dillon, J.G., Tatsumi, C.M., Tandingan, P.G. and Castenholz, R.W. (2002). Effect of environmental factors on the synthesis of scytonemin, a UV-screening pigment, in a cyanobacterium (Chroococcidiopsis sp.). Arch. Microbiol. 177: 322-331.
Donkor, V.A., Damian, H.A.K and Hader, D.P. (1993). Effects of tropical solar radiation on the motility of filamentous cyanobacteria. FEMS Microbiol. Ecol. 12: 143-148.
Dunlap, W.C. and Shick, J.M. (1998). Ultraviolet radiation-absorbing mycosporine-like amino acids in coral reef organisms: a biochemical and environmental perspective. J. Phycol. 34: 418-430.
Dunlap, W.C. and Yamamoto, Y. (1995). Small-molecule antioxidants in marine organisms: antioxi-dant activity of mycosporine-glycine. Com. Biochem. Physiol. 112: 105-114.
Edge, R., Mc Garvey, D.J. and Truscott, T.G. (1997). The carotenoids as antioxidants: a review. J. Photochem. Photobiol. B: Biol. 41: 189-200.
Ehling-Schulz, M. and Scherer, S. (1999). UV protection in cyanobacteria. Eur. J. Phycol. 34: 329-338.
Ehling-Schulz, M., Bilger, W. and Scherer, S. (1997). UV-B induced synthesis of photoprotective pig-ments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoc commune. J. Bacteriol. 179: 1940-1945.
Ehling-Schulz, M., Schulz, S., Wait, R., Görg, A., and Scherer, S. (2002). The UV-B stimulon of the terrestrial cyanobacterium Nostoc commune comprises early shock proteins and late acclimation proteins. Mol. Microbiol. 46: 827-843.
Favre-Bonvin, J., Bernillon, J., Salin, N. and Arpin, N. (1987). Biosynthesis of mycosporines: mycosporine glutaminol in Trichothecium roseum. Phytochemistry 26: 2509-2514.
Franklin, L.A. and Forster, R.M. (1997). The changing irradiance environment: consequences for marine macrophyte physiology. Eur. J. Phycol. 32: 207-232.
Fraser, P.J., Bouma, W.J., Forgan, B.W., Lehman, P. and Roy, C.R. (1992). The 1992 Antarctic ozone hole. Clean Air 26: 132-133.
Garcia-Pichel, F. and Belnap, J. (1996). Microenvironments and microscale productivity of cyanobac-terial desert crusts. J. Phycol. 32: 774-782.
Garcia-Pichel, F. and Castenholz, R.W. (1991). Characterization and biological implications of scy-tonemin, a cyanobacterial sheath pigment. J. Phycol. 27: 395-409.
Garcia-Pichel, F. and Castenholz, R.W. (1993). Occurrence of UV-absorbing mycosporine-like com-pounds among cyanobacterial isolates and an estimate of their screening capacity. Appl. Environ. Microbiol. 59: 163-169.
Garcia-Pichel, F. and Castenholz, R.W. (1994). On the significance of solar ultraviolet radiation for the ecology of microbial mats. In: L.J. Stal and P. Caumette (eds.) Microbial Mats, Structure, Development and Environmental significance, NATO ASI series, Springer-Verlag, Berlin, pp. 77-84.
Garcia-Pichel, F., Mechling, M. and Castenholz, R.W. (1994). Diel migrations of microorganisms within a benthic, hypersaline mat community. Appl. Environ. Microbiol. 60: 1500-1511.
Garcia-Pichel, F., Sherry, N.D. and Castenholz, R.W. (1992). Evidence for a UV-sunscreen role of the extracellular pigment scytonemin in the terrestrial cyanobacterium Chlorogloeopsis sp. Photochem. Photobiol. 56: 17-23.
Goetz, T., Windhoevel, U., Boeger, P. and Sandmann, G. (1999). Protection of photosynthesis against ultraviolet-B radiation by carotenoids in transformants of the cyanobacterium Synechococcus PCC7942. Plant Physiol. 120: 599-604.
He, Yu-Ying. and Häder, D.-P. (2002a). Reactive oxygen species and UV-B: effect on cyanobacteria. Photochem. Photobiol. Sci. 1: 729-736.
He, Yu-Ying. and Häder, D.-P. (2002b). UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-L-cysteine. J. Photochem. Photobiol. B: Biol. 66: 115-124.
Helm, R.F., Hung, Z., Edward, D., Leeson, H., Peery, W. and Potts, M. (2000). Structural character-ization of the released polysaccharide of desiccation tolerant Nostoc commune DRH-1. J. Bacteriol. 184: 974-982.
Hill, D., Keenan, T., Helm, R., Potts, M., Crowe, L. and Crowe, J. (1997). Extracellular polysaccha-ride of Nostoc commune (cyanobacteria) inhibits fusion of membrane vesicles during desiccation. J. Appl. Phycol. 9: 237-248.
Hughes, K.A. and Lawley, B. (2003). A novel Antarctic microbial endolithic community within gyp-sum crusts. Environ. Microbiol. 5: 555-565.
Huovinen, P., Gomez, I. and Lovengreen, C. (2006). A five-year study of solar ultraviolet radiation in southern Chile (39 degrees S): potential impact on physiology of coastal marine algae? Photochem. Photobiol. 82: 515-522.
Javor, B.J. and Castenholz, R.W. (1984). Productivity studies of microbial mats, Laguna Guerrero Negro, Mexico. In: Y. Cohen, H. Hulvorson and R.W. Castenholz (eds.) Microbial Mats: Stomatolites. Alan R. Liss. Inc, New York, pp. 149-170.
Jiang, H. and Qiu, B. (2005). Photosynthetic adaptation of a bloom-forming cyanobacterium Microcystis aeruginosa (cyanophyceae) to prolonged UV-B exposure. J. Phycol. 41: 983-992.
Karsten, U. (2002). Effects of salinity and ultraviolet radiation on the concentration of mycosporine-like amino acids in various isolates of the benthic cyanobacterium Microcoleus chthonoplastes. Phycol. Res. 50: 129-134.
Karsten, U. and Garcia-Pichel, F. (1996). Carotenoids and mycosporine-like amino acid compounds in members of the genus Microcoleus (cyanobacteria): a chemosystematic study. Syst. Appl. Microbiol. 19: 285-294.
Karsten, U., Maier, J. and Garcia-Pichel, F. (1998). Seasonality in UV-absorbing compounds of cyanobacterial mat communities from an intertidal mangrove flat. Aqua. Micro. Ecol. 16: 37-44.
Kerfeld, A.C. (2004). Water-soluble carotenoid proteins of cyanobacteria. Arch. Biochem. Biophys. 430: 2-9.
Knudsen, B.M., Jonch-Sorensen, H., Eriksen, P., Johansen, B.J. and Bodeker, G.E. (2005). UV radia-tion below an Arctic vortex with severe ozone depletion. Atmos. Chem. Phys. Discuss. 5: 4679-4700.
Kovacik, L. (2000). Cyanobacteria and algae as agents of biodeterioration of stone substrata of his-torical buildings and other cultural monuments, In: S. Choi, and M. Suh (eds.) Proceedings of the New Millenium International forum on conservation of cultural property. Kongju National University, Kongju, Korea, pp. 44-56.
Kruschell, C. and Castenholz, R.W. (1998). The effect of solar UV and visible irradiance on the ver-tical movements of cyanobacteria in microbial mats of hypersaline waters. FEMS Microbiol. Ecol. 27: 53-72.
Kumar, A., Sinha, R.P. and Häder, D.-P. (1996). Effects of UV-B on enzymes of nitrogen metabolism in the cyanobacterium Nostoc sp. J. Plant. Physiol. 148: 86-91.
Kumar, A., Tyagi, M.B., Jha, P.N., Srinivas, G. and Singh, A. (2003). Inactivation of cyanobacterial nitrogenase after to ultraviolet-B radiation. Curr. Microbiol. 46: 380-384.
Lakatos, M., Bilger, W. and Büdel, B. (2001). Carotenoid composition of terrestrial cyanobacteria: response to natural light conditions in open rock habitats in Venezuela. Eur. J. Phycol. 36: 367-375.
Lesser, M.P. and Stochaj, W.R. (1990). Photoadaptation and protection against active forms of oxy-gen in the symbiotic prokaryote prochloron-sp and its ascidian host. Appl. Environ. Microbiol. 56: 1530-1535.
Levine, E. and Thiel, T. (1987). UV-inducible DNA repair in the cyanobacteria Anabaena sp. J. Bacteriol. 169: 3988-3993.
Maeda, H., Sakuragi, Y., Bryant, D.A. and DellaPenna D. (2005). Tocopherols protect Synechocystis sp. strain PCC 6803 from lipid peroxidation. Plant Physiol. 138: 1422-1435.
Masaki, K., Dunlap, W.C., Yamamoto, Y., Karube, I., Larsen, R.M. and Matsukawa, R. (1996). A natural antioxidant and its production process. Toyo Suisan Kaisha Pty. Ltd. Japanese Patent Application No. 9604230.
Máté, Z., Sass, L., Szekeres, M., Vass, I. and Nagy, F. (1998). UV-B-induced differential transcription of psbA genes encoding the D1 protein of photosystem II in the cyanobacterium Synechocystis 6803. J. Biol. Chem. 273: 17439-17444.
Mazor, G., Kidron, G.J., Vonshak, A. and Abeliovich, A. (1996). The role of cyanobacterial exopolysaccharides in structuring desert microbial crusts. FEMS Microbiol. Ecol. 21: 121-130.
McKenzie, R.L., Bjorn, L.O., Bais, A. and Iiyas, M. (2003). Changes in biologically active ultraviolet radiation reaching the Earth’s surface. Photochem. Photobiol. Sci. 2: 5-15.
Miller, S.R., Wingard, C.E. and Castenholz, R.W. (1998). Effects of visible light and UV radiation on photosynthesis in a population of a hot spring cyanobacterium Synechococcus sp., subjected to high-temperature stress. Appl. Environ. Microbiol. 64: 3893-3899.
Miyake, C., Michihata, F. and Asada, K. (1991). Scavenging of hydrogen peroxide in prokaryotic and eukaryotic algae: acquisition of ascorbate peroxidase during the evolution of cyanobacteria. Plant Cell Physiol. 32: 33-43.
Ng, W.-O., Zentella, R., Wang, Y., Taylor, J.-S.A. and Pakrasi, H.B. (2000). A phrA, the major photore-activating factor in the cyanobacterium Synechocystis sp. strain PCC 6803 codes for a cyclobu-tanepyrimidine-dimer-specific DNA photolyase. Arch. Microbiol. 173: 412-417.
Oren, A. (1997). Mycosporine-like amino acids as osmotic solutes in a community of halophilic cyanobacteria. Geomicrobiol. J. 14: 231-240.
Oren, A., Kühl, M. and Karsten, U. (1995). An endoevaporitic microbial mat within a gypsum crust: zonation of phototrophs, photopigments, and light penetration. Mar. Ecol. Prog. Ser. 128: 151-159.
Pattanaik, B. and Adhikary, S.P. (2002). Blue-green algal flora at some archaeological sites and mon-uments of India. Feddes Repert. 113: 289-300.
Pattanaik, B. and Adhikary, S.P. (2004). Effects of UV-B irradiation on survival, spectral characteris-tics and nitrogenase activity of cyanobacteria from different habitats. Arch. Hydrobiol. Algol. Stud. 153: 159-173.
Portwich, A. and Garcia-Pichel, F. (2000). A novel prokaryotic UVB photoreceptor in cyanobac-terium Chlorogloeopsis PCC 6912. Photochem. Photobiol. 71: 493-498.
Potts, M. and Friedmann, E.I. (1981). Effects of water stress on cryptoendolithic cyanobacteria from hot desert rocks. Arch. Microbiol. 130: 267-271.
Potts, M. (1994). Desiccation tolerance of prokaryotes. Microbiol. Rev. 58: 755-805.
Proteau, P.J., Gerwick, W.H., Garcia-Pichel, F. and Castenholz, R. (1993). The structure of scytone-min, an ultraviolet sunscreen pigment from the sheaths of cyanobacteria. Experientia 49: 825-829.
Quesada, A. and Vincent, W.F. (1995). Strategies of adaptation by Antarctic cyanobacteria to ultra-violet radiation. Eur. J. Phycol. 32: 335-342.
Quesada, A., Vincent, W.F. and Lean, D.R.S. (1999). Community and pigment structure of Arctic cyanobacterial assemblages: the occurrence and distribution of UV-absorbing compounds. FEMS Microbiol. Ecol. 28: 315-323.
Ramsing, N.B. and Prufert-Bebout, L. (1994). Motility of Microcoleus chthonoplastes subjected to dif-ferent light intensities quantified by digital image analysis. In: L.J. Stal and P. Caumette (eds.) Microbial Mats, Structure, Development and Environmental Significance, NATO ASI series, Springer-Verlag, Berlin, pp. 183-191.
Reynolds, C.S., Oliver, R.L. and Walsby, A.E. (1987). Cyanobacterial dominance: the role of buoy-ancy regulation in dynamic lake environments. N. Z. J. Mar. Freshwater Res. 21: 379-390.
Rozema, J., Björn, L.O., Bornman, J.F., Gaberscik, A., Häder, D.-P., Trost, T., Germ, M., Klisch, M., Gröniger, A., Sinha, R.P., Lebert, M., He, Y.-Y., Buffoni-Hall, R., de Bakker, N.V.J., van de Staaij, J. and Meijkamp, B.B. (2002). The role of UV-B radiation in aquatic and terrestrial ecosystems-an experimental and functional analysis of the evolution of UV-absorbing com-pounds. J. Photochem. Photobiol. 66: 2-12.
Sass, L., Spetea, C., Mate, Z., Nagy, F. and Vass, I. (1997). Repair of UV-B induced damage of pho-tosystem II via de novo synthesis of the D1 and D2 reaction centre subunits in Synechocystis sp. PCC6803. Photo. Res. 54: 55-62.
Scherer, S., Ernst, A., Chen, T.W. and Böger, P. (1984). Rewetting of drought resistant blue-green algae: time course of water uptake and reappearance of respiration, photosynthesis and nitrogen fixation. Oecologia 62: 418-423.
Scherer, S., Chen, T.W. and Böger, P. (1988). A new UV-A/B protecting pigment in the terrestrial cyanobacterium Nostoc commune. Plant Physiol. 88: 1055-1057.
Schüßler, A., Meyer, T., Gehrig, H. and Kluge, M. (1997). Variations of lectin binding sites in extra-cellular glycoconjugates during the life cycle of Nostoc punctiforme, a potentially endosymbiotic cyanobacterium. Eur. J. Phycol. 32: 233-239.
Shirkly, B., Kovarcik, D.P., Wright, D.J., Wilmoth, G., Prickett, T.F., Helm, R.F., Gregory, E.M. and Potts, M. (2000). Active Fe-containing superoxide dismutase and abundant sodF mRNA in Nostoc commune (cyanobacteria) after years of desiccation. J. Bacteriol. 182: 189-197.
Sinha, R.P. and Häder, D.-P. (2002). UV-Induced DNA damage and repair: a review. Photochem. Photobiol. Sci. 1: 225-236.
Sørensen K.B., Canfield, D.E., Teske, A.P. and Oren, A. (2005). Community composition of a hyper-saline endoevaporitic microbial mat. Appl. Environ. Microbiol. 71: 7352-7365.
Stal, L.J. and Krumbein, W.E. (1985). Isolation and characterization of cyanobacteria from a marine microbial mat. Bot. Mar. 28: 351-365.
Steiger, S., Schaefer, L. and Sandmann, G. (1999). High-light-dependent upregulation of carotenoids and their antioxidative properties in the cyanobacterium Synechocystis PCC 6803. J. Photochem. Photobiol. B: Biology. 52: 14-18.
Tamaru, Y., Takani, Y., Yoshida, T. and Sakamoto, T. (2005). Crucial role of extracellular polysac-charides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune. Appl. Environ. Microbiol. 71: 7327-7333.
Tel-or, E., Huflejt, M.E. and Packer, L. (1986). Hydroperoxide metabolism in cyanobacteria. Arch. Biochem. Biophys. 246: 396-402.
Tomaselli, L. and Giovannetti, L. (1993). Survival of diazotrophic cyanobacteria in soil. World J. Microbiol. Biotech. 9: 113-116.
Tripathy, P., Roy, A., Anand, N. and Adhikary, S.P. (1999). Blue-green algal flora on the rock surface of temples and monuments of India. Feddes Repert. 110: 133-144.
Vass, I., Kirilovsky, D., Perewoska, I., Mate, Z., Nagy, F. and Etienne, A.L. (2000). UV-B radia-tion induced exchange of the D1 reaction centre subunits produced from the psbA2 and psbA3 genes in the cyanobacterium Synechocystis sp. PCC 6803. Eur. J. Biochem. 267: 2640-2648.
Viczián, A., Máté, Z., Nagy, F. and Vass, I. (2000). UV-B induced differential transcription of psbD genes encoding the D2 protein of Photosystem II in the cyanobacterium Synechocystis 6803. Photo. Res. 64: 257-266.
Vincent, W.F. and Quesada, A. (1994). Ultraviolet radiation effects on cyanobacteria: implications for Antarctic microbial ecosystems. In: C.S. Weiler and P.A. Penhale (eds.) Ultraviolet Radiation in Antarctica: Measurements and Biological Effects. Antarctic Research Series, American Geophysical Union, Washington, pp. 111-124.
Vincent, W.F. and Roy, S. (1993). Solar ultraviolet-B radiation and aquatic primary production: dam-age, protection and recovery. Environ. Rev. 1: 1-12.
Volkmann, M., Gorbushina, A.A., Kedar, L. and Oren, A. (2006). Structure of euhalothece-362, a novel red-shifted mycosporine-like amino acid, from a halophilic cyanobacterium (Euhalothece sp.). FEMS Microbiol. Lett. 258: 50-54.
Ward, D.M., Tayne, T.A., Anderson, K.L. and Bateson, M.M. (1987). Community structure and interactions among community members in hot spring cyanobacterial mats. Symp. Soc. Gen. Microbiol. 41: 179-210.
Wolfe-Simon, F., Grzebyk, D., Schofield O. and Falkowski, P.G. (2005). The role and evolution of superoxide dismutases in algae. J. Phycol. 41: 453-465.
Wright, D.J., Smith, S.C., Joardar, V., Scherer, S., Jervis, J., Warren, A., Helm, R.F. and Potts, M. (2005). UV irradiation and desiccation modulate the three-dimensional extracellular matrix of Nostoc commune (cyanobacteria). J. Biol. Chem. 280: 40271-40281.
Wu, H., Gao K., Villafane, V.E., Watanabe, T. and Helbling, E.W. (2005). Effects of solar UV radia-tion on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis. Appl. Environ. Microbiol. 71: 5004-5013.
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Pattanaik, B., Schumann, R., Karsten, U. (2007). Effects of Ultraviolet Radiation on Cyanobacteria and their Protective Mechanisms. In: Seckbach, J. (eds) Algae and Cyanobacteria in Extreme Environments. Cellular Origin, Life in Extreme Habitats and Astrobiology, vol 11. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6112-7_2
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