Abstracts
This chapter is devoted to methods based on suppression polymerase chain reaction (PCR) effect (cDNA library construction starting from a small amount of total RNA; suppression subtractive hybridization, SSH; ordered differential display, ODD; Marathon cDNA RACE; genome walking; variants of coincidence cloning, CC; normalization of cDNA libraries; multiplex PCR, mPCR; to in vitro cloning). Taken together, these approaches allow one to analyze complex DNA samples, from searching sequences of interest to determining complete structures of the respective genes.
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© 2007 Springer
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Lukyanov, S.A., Lukyanov, K.A., Gurskaya, N.G., Bogdanova, E.A., Buzdin, A.A. (2007). Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications. In: Buzdin, A.A., Lukyanov, S.A. (eds) Nucleic Acids Hybridization Modern Applications. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6040-3_2
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DOI: https://doi.org/10.1007/978-1-4020-6040-3_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-6039-7
Online ISBN: 978-1-4020-6040-3
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