Skip to main content

Abstract

Debaryomyces hansenii is a very peculiar spoilage microorganism: this yeast shows a good performance under concentrations of sodium chloride which prevent growth of most microorganisms. Here we report aspects of this behaviour and present data which support the theory that the salt loving nature of D. hansenii can be explained by the capability of the membrane potassium carriers to transport potassium into the cells, even in the presence of high concentrations of sodium.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Adler L, Blomberg A, Nilsson A. “Glycerol metabolism and osmoregulation in salt-tolerant yeast Debaryomyces hansenii”, Journal of Bacteriology, 162, pp. 300–306, 1985.

    Google Scholar 

  2. Almagro A, Prista C, Benito B, Loureiro-Dias MC, Ramos J. “Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii”. Journal of Bacteriology, 183, pp. 3251–3255, 2001.

    Article  Google Scholar 

  3. Almagro A, Prista C, Castro S, Quintas C, Madeira-Lopes A, Ramos J, Loureiro-Dias MC. “Effects of salts on Debaryomyces hansenii and Saccharomyces cerevisiae under stress conditions”. International Journal of Food Microbiology, 56, pp. 191–197, 2000.

    Article  Google Scholar 

  4. André L, Nilsson A, Adler L. “The role of glycerol in osmotolerance of the yeast Debaryomyces hansenii”. Journal of General Microbiology, 134, pp. 669–677, 1988.

    Google Scholar 

  5. Bañuelos MA, Ramos J, Calero F, Braun V, Potier, S. “Cation/H+ antiporters mediate potassium and sodium fluxes in Pichia sorbitophila. Cloning of the PsNHA1 and PsNHA2 genes and expression in Saccharomyces cerevisiae”. Yeast, 19, pp. 1365–1372, 2002.

    Article  Google Scholar 

  6. Deak T, Beuchat LR. Handbook of Food Spoilage Yeasts, Boca Raton, CRC Press, 1996.

    Google Scholar 

  7. Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, De Montigny J, Marck C, Neuveglise C, Talla E, Goffard N, Frangeul L, Aiglem M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich JM, Beyne E, Bleykasten C, Boisrame A, Boyer J, Cattolico L, Confanioleri F, De Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, Groppi A, Hantraye F, Hennequin C, Jauniaux N, Joyet P, Kachouri R, Kerrest A, Koszul R, Lemaire M, Lesur I, Ma L, Muller H, Nicaud JM, Nikolski M, Oztas S, Ozier-Kalogeropoulos O, Pellenz S, Potier S, Richard GF, Straub ML, Suleau A, Swennen D, Tekaia F, Wesolowski-Louvel M, Westhof E, Wirth B, Zeniou-Meyer M, Zivanovic I, Bolotin-Fukuhara M, Thierry A, Bouchier C, Caudron B, Scarpelli C, Gaillardin C, Weissenbach J, Wincker P, Souciet JL. “Genome evolution in yeasts”. Nature, 430, pp. 35–44, 2004.

    Article  Google Scholar 

  8. Gonzalez-Hernandez JC, Cardenas-Monroy CA, Peña A. “Sodium and potassium transport in the halophilic yeast Debaryomyces hansenii”. Yeast, 21, pp. 403–412, 2004.

    Article  Google Scholar 

  9. Gori K, Mortensen HD, Arneborg N, Jespersen L. “Expression of the GPD1 and GPP2 orthologues and glycerol retention during growth of Debaryomyces hansenii at high NaCl concentrations”. Yeast, 22, pp. 1213–22, 2005.

    Article  Google Scholar 

  10. Haro, R., M.A. Bañuelos, F.J. Quintero, F. Rubio, and A. Rodríguez-Navarro. Genetic basis of sodium exclusion and sodium tolerance in yeast. A model for plants. Physiology Plantarum, 89, pp. 868–874, 1993.

    Article  Google Scholar 

  11. Hirasawa T, Nakakura Y, Yoshikawa K, Ashitani K, Nagahisa K, Furusawa C, Katakura Y, Shimizu H, Shioya S. “Comparative analysis of transcriptional responses to saline stress in the laboratory and brewing strains of Saccharomyces cerevisiae with DNA microarray”. Applied Microbiology and Biotechnology, 70, pp. 346–57, 2006.

    Article  Google Scholar 

  12. Hohmann S. “Osmotic stress signalling and osmoadaptation in yeasts”. Microbiology and Molecular Biology Reviews. 66, pp. 300–372, 2002.

    Article  Google Scholar 

  13. Norkrans B, Kylin A. “Regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeasts”. Journal of Bacteriology, 100, pp. 836–845, 1969.

    Google Scholar 

  14. Prista C, Almagro A, Loureiro-Dias MC, Ramos J. “Kinetics of cation movements in Debaryomyces hansenii”.Folia Microbiol (Praha), 43, pp. 212–4, 1998.

    Google Scholar 

  15. Prista C, Almagro A, Loureiro-Dias MC, Ramos J. “Physiological basis for the high salt tolerance of Debaryomyces hansenii”. Applied and Environental Microbiology, 63, pp. 4005–4009, 1997.

    Google Scholar 

  16. Prista C, González-Hernández JC, Ramos J, Loureiro-Dias MC “Potassium transpot systems in Debaryomyces hansenii”. Yeast, 22, pp. S184, 2005.

    Google Scholar 

  17. Rengpipat S, Lowe SE, Zeikus JG. “Effect of extreme salt concentrations on the physiology and biochemistry of Halobacteroides acetoethylicus”. Journal of Bacteriology, 170, pp. 3065–3071, 1988.

    Google Scholar 

  18. Rodríguez-Navarro A. “Potassium transport in fungi and plants”. Biochimica Biophysica Acta, 1469, pp. 1–30, 2000.

    Google Scholar 

  19. Wadskog I, Adler L. “Ion homeostasis in Saccharomyces cerevisiae under NaCl stress”. Yeast stress responses. In Topics in Current Genetics, Hohmann S. and Mager WH, Eds), pp. 201–239, Springer, 2003.

    Google Scholar 

  20. Watanabe Y, Miwa S, Tamai Y. “Characterization of Na+/H(+)-antiporter gene closely related to the salt-tolerance of yeast Zygosaccharomyces rouxii”. Yeast, 11, pp.829–38, 1995.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this paper

Cite this paper

Prista, C., Loureiro-Dias, M.C. (2007). Debaryomyces Hansenii, a Salt Loving Spoilage Yeast. In: Pereira, M.S. (eds) A Portrait of State-of-the-Art Research at the Technical University of Lisbon. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5690-1_28

Download citation

  • DOI: https://doi.org/10.1007/978-1-4020-5690-1_28

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-5689-5

  • Online ISBN: 978-1-4020-5690-1

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics