The terms recombinant DNA technology, DNA cloning, molecular cloning, or gene cloning all refer to use of molecular techniques to select a specific sequence or sequences of DNA from an organism and transfer it into another organism to code for or alter specific traits. Thus, recombinant DNA technology provides a powerful molecular tool that enables scientists to engineer sequences of DNA.
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- ampr:
-
ampicillin resistance gene
- ATP:
-
adenosine triphosphate
- BAC:
-
bacterial artificial chromosome
- Ca2+:
-
calcium ion
- CaCl2:
-
calcium chloride
- cDNA:
-
complementary DNA
- Cl–:
-
chlorine ion
- CO2:
-
carbon dioxide
- DNA:
-
deoxyribonucleic acid
- ES:
-
embryonic stem cells
- GH:
-
growth hormone
- HAC:
-
human artificial chromosome
- HPO4 2–:
-
monohydrogen phosphate ion
- ICM:
-
inner cell mass
- IPTG:
-
isopropyl-β-D thiogalactopyranoside
- K+:
-
potassium ion
- LacZ′:
-
β-galactosidase gene of E. coli
- MCS:
-
multiple cloning sites also called polylinkers
- Mg2+:
-
magnesium ion
- mRNA:
-
messenger RNA
- Na+:
-
sodium ion
- NH4+:
-
ammonium
- RNA:
-
ribonucleic acid
- SO4 2–:
-
sulfate ion
- YAC:
-
yeast artificial chromosome
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Chedrese, P.J. (2009). Recombinant DNA Technology. In: Chedrese, P. (eds) Reproductive Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-88186-7_7
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