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Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM

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Biological Low-Voltage Scanning Electron Microscopy

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References

  • Albrecht RM, Simmons SR, and Pawley JB (1993) Correlative video-enhanced light microscopy, high voltage transmission electron microscopy, and field emission scanning electron microscopy for the localization of colloidal-gold labels. In: Immunocytochemistry: A Practical Approach. J.E. Beesley, ed. Oxford University Press, Oxford. pp 151–176.

    Google Scholar 

  • Albrecht RM, and MacKenzie AP (1975) Cultured and Free Living Cells. In Principles and Techniques for Scanning Electron Microscopy: Biological Applications. M.A. Hayat ed. Van Nostrand Reinhold, NY, 3:109–153

    Google Scholar 

  • Albrecht RM and Meyer DA (2002) All that glitters is not gold: Approaches to labeling for EM. Microscopy and Microanlysis 8, suppl.2:194–195.

    Google Scholar 

  • Albrecht RM and Wetzel B (1979) Ancillary methods for biological SEM. Scan. Elec. Microsc. Vol. III:203–222.

    Google Scholar 

  • Alivisatos AP, Gu W, and Larabell C (2005) Quantum dots as cellular probes. Annual Review of Biomedical Engineering 7(1), 55–76.

    Article  PubMed  CAS  Google Scholar 

  • Bleher R, Meyer DA, and Albrecht RM (2004) Multiple labeling for EM using colloidal particles of gold, palladium, and platinum as markers. Microscopy and Microanalysis 10, Suppl.-2:158–159.

    Google Scholar 

  • Bleher R, Meyer DA, and Albrecht RM (2005) High-resolution multiple labeling for immuno-EM applying metal colloids and energy filtering transmission electron microscopy (EFTEM). Microscopy and Microanalysis 11, Suppl.-2:1100–1101.

    Google Scholar 

  • Bleher R, Kandela I, Meyer DA, and Albrecht RM (2007). Immuno-EM using colloidal metal nanoparticles and electron spectroscopic imaging for co-localization at high spatial resolution. J. Micros. In Press.

    Google Scholar 

  • Danscher G (1981) Localization of gold in biological tissue: a photochemical method for light and electron microscopy. Histochemistry 71:81

    Google Scholar 

  • Deerinck TJ, Giepmans BNG, and Ellisman MH (2005) Quantum Dots as Cellular Probes for Light and Electron Microscopy. Microscopy and Microanalysis 11, Suppl.-2:914–915.

    Google Scholar 

  • Eppell SJ, Simmons SR, Albrecht RM, and Marchant RE (1995) Cell surface receptors and proteins on platelet membranes imaged by scanning force microscopy using immunogold contrast enhancement. Biophysical Journal 68:671–680.

    Article  PubMed  CAS  Google Scholar 

  • Faraday M (1857) Experimental relations of gold (and other metals) to light. Philosophical Transactions of the Royal Society of London. 147:145–181.

    Article  Google Scholar 

  • Frens G (1973) Nature Physical Science. 241:20–22

    CAS  Google Scholar 

  • Gelles J, Schnapp BJ, and Sheetz MP (1988) Tracking kinesin-driven movements with nanometre-scale precision, Nature 331:450–453.

    Article  PubMed  CAS  Google Scholar 

  • Goodman SL, Park K, and Albrecht RM (1991) A correlative approach to colloidal-gold labeling with video-enhanced light microscopy, low-voltage scanning electron microscopy, and high-voltage electron microscopy. In: Colloidal-Gold: Principles, Methods, and Applications. Vol. 3. M.A. Hayat., ed. Academic Press, Inc., San Diego. pp 369–409.

    Google Scholar 

  • Goodman SL, and Albrecht RM (1996) Cell Surface Interactions: the Blood Platelet as a Paradigm. Ch 15 Interfacial Phenomena and Bioproducts, J.L. Brash and P.W. Wojciechowski (eds.), Marcel Dekker Inc., New York, NY publishers, pp. 485–506.

    Google Scholar 

  • Hainfeld JF, Powell RD, Stein JK, Hacker GW, Hauser-Kronberger C, Cheung ALM, and Schofer C (1999) Gold-based autometallography; Proc. 57 th Ann. Mtg., Micros. Soc. Amer.; G. W. Bailey, W. G. Jerome, S. McKernan, J. F. Mansfield, and R. L. Price (Eds.); Springer-Verlag: New York, NY; 486–487.

    Google Scholar 

  • Handley DA (1989) The development and application of colloidal-gold as a microscopic probe. In: Colloidal-Gold: Principles, Methods, and Applications. Vol. 1. M.A. Hayat., ed. Academic Press, Inc., San Diego. pp 13–32.

    Google Scholar 

  • Henglein A (2000) Colloidal palladium nanoparticles: reduction of Pd (II) by H2; PdcoreAushellAgshell particles. The Journal of Physical Chemistry B. 104(29):6683–6685.

    Article  CAS  Google Scholar 

  • Hodak JH, Henglein A, and Harland GV (2001) Tuning the spectral and temporal response in PtAu core-shell nanoparticles. Journal of Chemical Physics. 114(6):2760–2765.

    Article  CAS  Google Scholar 

  • Joy DC (1989) Control of charging in low-voltage SEM. Scanning 11-1:1–4.

    Google Scholar 

  • Kaiser M, Heintz J, Kandela I, and Albrecht RM (2007) Tumor cell death induced by membrane melting via Inductively Heated Core/Shell Nanoparticles. Microscopy and Microanalysis, 13, supp-2:18–19.

    Google Scholar 

  • Kandela IK, Bleher R, and Albrecht RM (2004), Correlative Labeling Studies in Light and Electron Microscopy. Micros&Microanaly 10, suppl-2:1212-1213. 2004.

    Google Scholar 

  • Kandela IK, Bleher R, and Albrecht RM (2005) Correlative Immunolabeling on Etched Epon Samples. Microscopy and Microanalysis 11, supp-2:1098–1099.

    Google Scholar 

  • Kandela IK and Albrecht RM (2007) Fluorescence quenching by colloidal heavy metal nanoparticles: Implications for correlative fluorescence and electron microscopy studies. Scanning 21:152–161.

    Article  Google Scholar 

  • Kandela IK, Bleher R, and ALbrecht RM (2007a) Multiple correlative immunolabeling for light and electron microscopy using fluorphores and colloidal metal particles. J. Histochem. Cytochem. In Press.

    Google Scholar 

  • Kandela IK, Bleher R, and Albrecht RM (2007b) Immunolabeing for correlative light and electron microscopy on ultrathin cryosections. Micros. and Microanal. In Press.

    Google Scholar 

  • Kandela IK (2006) Development of Metal Nanoparticle Immunoconjugates for Correlative Labeling in Light and Electron Microscopy and as Active Targeted Delivery Systems. PhD Thesis, University of Wisconsin, Madison, WI

    Google Scholar 

  • Kachar B (1985) Asymmetric Illumination Contrast: A Method of Image Formation for Video Light Microscopy. Science 227:766–768.

    Article  PubMed  CAS  Google Scholar 

  • Koeck PJB and Leonard KR (1996) Improved Immuno Double Labeling for Cell and Structural Biology. Micron 27:57–165.

    Article  Google Scholar 

  • Lai QJ, Simmons SR, Albrecht RM, and Cooper SL (1992) Protein and cell adhesion to block polymer microdomains, Transactions of the Society for Biomaterials.

    Google Scholar 

  • Lai QJ (1992) The role of proteins and cellular interactions on the blood compatibility of polymers and other artificial surfaces. PhD Thesis, University of Wisconsin, Madison, WI

    Google Scholar 

  • Meyer DA and Albrecht RM (2002) Size selective synthesis of colloidal platinum nanoparticles for use as high-resolution EM labels. Microscopy and Microanalysis 8, Suppl.-2:124–125.

    Google Scholar 

  • Meyer DA and Albrecht RM (2003), Sodium ascorbate method for the synthesis of colloidal palladium particles of different sizes. Microscopy and Microanalysis 9-Suppl.-2:1190–1191.

    Google Scholar 

  • Meyer DA, Bleher R, Kandela IK, Oliver JA, and Albrecht RM (2006) The development of alternative markers for transmission electron microscopy and correlative transmission electron and light microscopies. Microscopy and Microanalysis 12, Suppl.-2:32–33.

    Article  Google Scholar 

  • Mühlpfordt H (1982) The preparation of colloidal-gold particles using tannic acid as an additional reducing agent. Experientia 38:1127–1128.

    Article  Google Scholar 

  • Nisman R, Dellaire G, Ren Y, Li R, and Bazett-Jones DP (2004) Application of Quantum Dots as Probes for Correlative Fluorescence, Conventional, and Energy-filtered Transmission Electron Microscopy. J. Histochem. Cytochem. 52–1:13–18.

    Google Scholar 

  • Park K, Simmons SR, and Albrecht RM (1988) Surface characterization of biomaterials by immunogold staining - Quantitative analysis. In Biotechnology and Bioapplications of Colloidal-GoldAlbrecht RM and Hodges GM eds. Scanning Microscopy International, Chicago IL. pp 41–52.

    Google Scholar 

  • Park K, Park H, and Albrecht RM (1989), Factors affecting the staining with colloidal-gold. In: Colloidal-Gold: Principles, Methods, and Applications. Vol. 1. M.A. Hayat., ed. Academic Press, Inc., San Diego. pp 489–518.

    Google Scholar 

  • Pawley JB,Albrecht RM (1988), Imaging colloidal-gold labels in LVSEM, Scanning 10:184–189

    CAS  Google Scholar 

  • Pawley JB and Erlandsen SL (1989) The case for low voltage high-resolution scanning electron microscopy of biological samples. Scanning Microscopy 3, Suppl-3:163–178

    CAS  Google Scholar 

  • Roth J, (1982) The preparation of protein A-gold complexes with 3nm and 15nm gold particles and their use in labelling multiple antigens on ultra-thin sections. Histochemical Journal. 14–5:791–801.

    Article  Google Scholar 

  • Scopsi L (1989) Silver Enhanced Colloidal-Gold Method.,In: Colloidal-Gold: Principles, Methods, and Applications. Vol. 1. M.A. Hayat., ed. Academic Press, Inc., San Diego. pp 251–295.

    Google Scholar 

  • Simmons SR and Albrecht RM (1989) Probe size and bound label conformation in colloidal gold-ligand labels and gold-immunolabels. Scanning Microscopy 3-Suppl. 3:27–34.

    Google Scholar 

  • Simmons SR, Pawley JB, and Albrecht RM (1990) Optimizing parameters for correlative immunogold localization by video-enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy. J. Histochem. Cytochem. 38:1781–1785.

    PubMed  CAS  Google Scholar 

  • Sims P, Albrecht RM, Pawley JB, Centonze V, Deerinck T, and Hardin J (2006) When Light Microscope Resolution Is Not Enough: Correlational Light Microscopy and Electron Microscopy, in Handbook of Biological Confocal Microscopy, 3rd edition, (Pawley, JB, ed.), Springer, NY, 49:846–860.

    Google Scholar 

  • Slot JW and Geuze HJ (1985) A new method of preparing gold probes for multiple-labeling cytochemistry. European Journal of Cell Biology. 38–1:87–93.

    Google Scholar 

  • Stierhof Y-D, Humbel BM, and Schwartz H (1991) J. Electron. Micros. Tech 17: 336.

    Article  CAS  Google Scholar 

  • Turkevich J, Stevenson PC, and Hillier J (1951) A study of the nucleation and growth processes in the synthesis of colloidal-gold. Discussions of the Faraday Society. 11:55–75.

    Article  Google Scholar 

  • Verwey EJW and Overbeek JThG (1948) Theory of the Stability of Lyophobic Colloids. Elsevier Publishing Co., Inc. New York.

    Google Scholar 

  • Voigt J, and Heumann J (1928) Die herstellung schutzkolloidfreier, gleichteiliger silberhydrosole. Zeitschrift für Anorganische Chemie. 169(1–3):140–150.

    Google Scholar 

  • Wetzel BK and Albrecht RM (1989) The Evolution of Correlative Techniques for Electron Microscopy – An Overview In: The Science of Biological Specimen Preparation. R.M. Albrecht and R.L. Ornberg (eds). Scanning Microscopy Supplement 3:1–6.

    Google Scholar 

  • Zsigmondy R (1906) Zeitschrift für Physikalische Chemie. 56:65.

    CAS  Google Scholar 

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Authors
  1. Ralph Albrecht
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  2. Daryl Meyer
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Editor information

Editors and Affiliations

  1. Department of Veterinary Pathobiology, University of Missouri-Columbia, 1600 E. Rollins Street, Columbia, MO 65211, USA

    Heide Schatten

  2. Department of Zoology, University of Wisconsin, 250 N. Mills Street, Madison, WI 53706, USA

    James B. Pawley

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Albrecht, R., Meyer, D. (2008). Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM. In: Schatten, H., Pawley, J.B. (eds) Biological Low-Voltage Scanning Electron Microscopy. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72972-5_6

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