Abstract
Iron oxide particles have been used for molecular and cellular magnetic resonance imaging since the 1980s. Interestingly, early research in these fields used particles in the micron size range. After a long disappearance, the utility of micron-sized iron oxide particles, or MPIOs, has been rediscovered, largely due to their improved construction and stability. Due to the very high iron content of single MPIOs, their use as a cell-tracking agent has enabled the detection of single particles in single cells, the detection of single cells in vivo, and the ability to label cells directly in vivo. This chapter begins by discussing the physical and magnetic properties of MPIOs and compares them to the more commonly used iron oxide particles, USPIOs and SPIOs. Next, the unique properties of MPIOs as an MRI agent are illustrated, with examples of MRI experiments on the particles themselves, and on cells labeled with MPIOs. The chapter then reviews work on various applications of cell therapy, using cells labeled with MPIOs, with a section on the detection of single cells in vivo. Lastly, there is a presentation of work using MPIOs to directly label cells in vivo.
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References
Ahrens, E.T., Feili-Hariri, M., Xu, H., Genove, G., Morel, P.A., 2003. Receptor-mediated endocytosis of iron-oxide particles provides efficient labeling of dendritic cells for in vivo MR imaging. Magn Reson Med 49, 1006–1013.
Arbuthnott, G.W., Dunnett, S.B., 1990. Indentification of grafted neurons with fluorescent-labelled microspheres. In: Dunnett, S.B., Richards, S.J. (Eds.), Progress in Brain Research. Elsevier Science Publisher B.V., Amsterdam, pp. 385–390.
Artemov, D., Bhujwalla, Z.M., Bulte, J.W., 2004. Magnetic resonance imaging of cell surface receptors using targeted contrast agents. Curr Pharm Biotechnol 5, 485–494.
Benderbous, S., Corot, C., Jacobs, P., Bonnemain, B., 1996. Superparamagnetic agents: physicochemical characteristics and preclinical imaging evaluation. Acad Radiol 3 Suppl 2, S292–S294.
Bowen, C.V., Zhang, X., Saab, G., Gareau, P.J., Rutt, B.K., 2002. Application of the static dephasing regime theory to superparamagnetic iron-oxide loaded cells. Magn Reson Med 48, 52–61.
Bulte, J.W., Hoekstra, Y., Kamman, R.L., Magin, R.L., Webb, A.G., Briggs, R.W., Go, K.G., Hulstaert, C.E., Miltenyi, S., The, T.H., 1992. Specific MR imaging of human lymphocytes by monoclonal antibody-guided dextran-magnetite particles. Magn Reson Med 25, 148–157.
Callaghan, P.T., 1993. Principles of nuclear magnetic resonance microscopy. Clarendon Press, Oxford, England.
Corot, C., Petry, K.G., Trivedi, R., Saleh, A., Jonkmanns, C., Le Bas, J.F., Blezer, E., Rausch, M., Brochet, B., Foster-Gareau, P., Baleriaux, D., Gaillard, S., Dousset, V., 2004. Macrophage imaging in central nervous system and in carotid atherosclerotic plaque using ultrasmall superparamagnetic iron oxide in magnetic resonance imaging. Invest Radiol 39, 619–625.
Cunningham, C.H., Arai, T., Yang, P.C., McConnell, M.V., Pauly, J.M., Conolly, S.M., 2005. Positive contrast magnetic resonance imaging of cells labeled with magnetic nanoparticles. Magn Reson Med 53, 999–1005.
Deans, A.E., Wadghiri, Y.Z., Turnbull, D.H., 2006. MRI of neural progenitor migration in the developing mouse brain. Proc 14th ISMRM, abstract #355.
Dick, A.J., Guttman, M.A., Raman, V.K., Peters, D.C., Pessanha, B.S., Hill, J.M., Smith, S., Scott, G., McVeigh, E.R., Lederman, R.J., 2003. Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in Swine. Circulation 108, 2899–2904.
Dodd, S.J., Williams, M., Suhan, J.P., Williams, D.S., Koretsky, A.P., Ho, C., 1999. Detection of single mammalian cells by high-resolution magnetic resonance imaging. Biophys J 76, 103–109.
Doetsch, F., Alvarez-Buylla, A., 1996. Network of tangential pathways for neuronal migration in adult mammalian brain. Proc Natl Acad SciUSA 93, 14895–14900.
Dunning, M.D., Kettunen, M.I., Ffrench, C.C., Franklin, R.J., Brindle, K.M., 2006. Magnetic resonance imaging of functional Schwann cell transplants labelled with magnetic microspheres. Neuroimage 31, 172–180.
Foster-Gareau, P., Heyn, C., Alejski, A., Rutt, B.K., 2003. Imaging single mammalian cells with a 1.5 T clinical MRI scanner. Magn Reson Med 49, 968–971.
Frank, J.A., Miller, B.R., Arbab, A.S., Zywicke, H.A., Jordan, E.K., Lewis, B.K., Bryant, L.H., Jr., Bulte, J.W., 2003. Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology 228, 480–487.
Frank, J.A., Zywicke, H., Jordan, E.K., Mitchell, J., Lewis, B.K., Miller, B., Bryant, L.H., Jr., Bulte, J.W., 2002. Magnetic intracellular labeling of mammalian cells by combining (FDA-approved) superparamagnetic iron oxide MR contrast agents and commonly used transfection agents. Acad Radiol 9 Suppl 2, S484–S487.
Hawrylak, N., Ghosh, P., Broadus, J., Schlueter, C., Greenough, W.T., Lauterbur, P.C., 1993. Nuclear magnetic resonance (NMR) imaging of iron oxide-labeled neural transplants. Exp Neurol 121, 181–192.
Heyn, C., Ronald, J.A., Mackenzie, L.T., MacDonald, I.C., Chambers, A.F., Rutt, B.K., Foster, P.J., 2006. In vivo magnetic resonance imaging of single cells in mouse brain with optical validation. Magn Reson Med 55, 23–29.
Hill, J.M., Dick, A.J., Raman, V.K., Thompson, R.B., Yu, Z.X., Hinds, K.A., Pessanha, B.S., Guttman, M.A., Varney, T.R., Martin, B.J., Dunbar, C.E., McVeigh, E.R., Lederman, R.J., 2003. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation 108, 1009–1014.
Hinds, K.A., Hill, J.M., Shapiro, E.M., Laukkanen, M.O., Silva, A.C., Combs, C.A., Varney, T.R., Balaban, R.S., Koretsky, A.P., Dunbar, C.E., 2003. Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood 102, 867–872.
Hogemann, D., Josephson, L., Weissleder, R., Basilion, J.P., 2000. Improvement of MRI probes to allow efficient detection of gene expression. Bioconjug Chem 11, 941–946.
Jiang, Q., Zhang, Z.G., Ding, G.L., Zhang, L., Ewing, J.R., Wang, L., Zhang, R., Li, L., Lu, M., Meng, H., Arbab, A.S., Hu, J., Li, Q.J., Pourabdollah Nejad, D.S., Athiraman, H., Chopp, M., 2005. Investigation of neural progenitor cell induced angiogenesis after embolic stroke in rat using MRI. Neuroimage 28, 698–707.
Josephson, L., Tung, C.H., Moore, A., Weissleder, R., 1999. High-efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates. Bioconjug Chem 10, 186–191.
Lauterbur, P.C., Bernardo, M.L., Jr., Mendonca Dias, M.H., Heldman, A.W., 1986. Microscopic NMR imaging of the magnetic fields around magnetite particles. Proc 5th SMRM, 229.
Perez, J.M., Josephson, L., O’Loughlin, T., Hogemann, D., Weissleder, R., 2002a. Magnetic relaxation switches capable of sensing molecular interactions. Nat Biotechnol 20, 816–820.
Perez, J.M., O’Loughin, T., Simeone, F.J., Weissleder, R., Josephson, L., 2002b. DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA-cleaving agents. J Am Chem Soc 124, 2856–2857.
Ponder, K.P., Gupta, S., Leland, F., Darlington, G., Finegold, M., DeMayo, J., Ledley, F.D., Chowdhury, J.R., Woo, S.L., 1991. Mouse hepatocytes migrate to liver parenchyma and function indefinitely after intrasplenic transplantation. Proc Natl Acad Sci USA 88, 1217–1221.
Raynal, I., Prigent, P., Peyramaure, S., Najid, A., Rebuzzi, C., Corot, C., 2004. Macrophage endocytosis of superparamagnetic iron oxide nanoparticles: mechanisms and comparison of ferumoxides and ferumoxtran-10. Invest Radiol 39, 56–63.
Renshaw, P.F., Owen, C.S., Evans, A.E., Leigh, J.S., Jr., 1986. Immunospecific NMR contrast agents. Magn Reson Imaging 4, 351–357.
Rodriguez, O., Fricke, S., Chien, C., Dettin, L., Vanmeter, J., Shapiro, E., Dai, H.N., Casimiro, M., Ileva, L., Dagata, J., Johnson, M.D., Lisanti, M.P., Koretsky, A., Albanese, C., 2006. Contrast-enhanced in vivo imaging of breast and prostate cancer cells by MRI. Cell Cycle 5, 113–119.
Saini, S., Stark, D.D., Hahn, P.F., Bousquet, J.C., Introcasso, J., Wittenberg, J., Brady, T.J., Ferrucci, J.T., Jr., 1987a. Ferrite particles: a superparamagnetic MR contrast agent for enhanced detection of liver carcinoma. Radiology 162, 217–222.
Saini, S., Stark, D.D., Hahn, P.F., Wittenberg, J., Brady, T.J., Ferrucci, J.T., Jr., 1987b. Ferrite particles: a superparamagnetic MR contrast agent for the reticuloendothelial system. Radiology 162, 211–216.
Shapiro, E.M., Gonzalez-Perez, O., Garcia-Verdugo, J.M., Alvarez-Buylla, A., Koretsky, A.P., 2006a. Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. Neuroimage. 32, 1150–1157.
Shapiro, E.M., Medford-Davis, L.N., Dunbar, C.E., Koretsky, A.P., 2006b. Antibody mediated cell labeling of peripheral T cells with micron sized iron oxide particles (MPIOs) allows single cell detection by MRI. Proc 14th ISMRM, abstract # 1868.
Shapiro, E.M., Medford-Davis, L.N., Fahmy, T., Dunbar, C.E., Koretsky, A.P., 2007. Antibody-red cell labeling of peripheral T cells with micron-Sized iron oxide particles 7. Micron-Sized Iron Oxide Particles for Cellular Imaging 161 (MPIOs) allows single cell detection by MRI, Contest Media and Molecular Imaging, 2, 53.
Shapiro, E.M., Sharer, K., Skrtic, S., Koretsky, A.P., 2006c. In vivo detection of single cells by MRI. Magn Reson Med 55, 242–249.
Shapiro, E.M., Skrtic, S., Koretsky, A.P., 2005. Sizing it up: cellular MRI using micron-sized iron oxide particles. Magn Reson Med 53, 329–338.
Shapiro, E.M., Skrtic, S., Sharer, K., Hill, J.M., Dunbar, C.E., Koretsky, A.P., 2004. MRI detection of single particles for cellular imaging. Proc Natl Acad Sci USA 101, 10901–10906.
Tanimoto, A., Pouliquen, D., Kreft, B.P., Stark, D.D., 1994. Effects of spatial distribution on proton relaxation enhancement by particulate iron eoxide. J Magn Reson Imaging 4, 653–657.
Tracy, K., Yin, J.J., Munasinghe, J., Shapiro, E.M., Koretsky, A.P., Kelly, K., 2006. The role of VEGF in growth of brain metastases from single cells as visualized by contrast enhanced MRI. Proc 14th ISMRM, abstract # 466.
Vreys, R., Peleman, C., Geraerts, M., De Cuyper, M., Debyser, Z., Baekelandt, V., Ven der Linden, A., 2006. Validation of magnetoliposomes as MR contrast agents for in situ labeling of endogenous neural progenitor cells in the mouse brain. Proc 14th ISMRM, abstract #356.
Weisskoff, R.M., Zuo, C.S., Boxerman, J.L., Rosen, B.R., 1994. Microscopic susceptibility variation and transverse relaxation: theory and experiment. Magn Reson Med 31, 601–610.
Wu, Y.L., Ye, Q., Foley, L.M., Hitchens, T.K., Sato, K., Williams, J.B., Ho, C., 2006. In situ labeling of immune cells with iron oxide particles: an approach to detect organ rejection by cellular MRI. Proc Natl Acad Sci USA 103, 1852–1857.
Yeh, T.C., Zhang, W., Ildstad, S.T., Ho, C., 1993. Intracellular labeling of T-cells with superparamagnetic contrast agents. Magn Reson Med 30, 617–625.
Zhang, R.L., Zhang, L., Zhang, Z.G., Morris, D., Jiang, Q., Wang, L., Zhang, L.J., Chopp, M., 2003. Migration and differentiation of adult rat subventricular zone progenitor cells transplanted into the adult rat striatum. Neuroscience 116, 373–382.
Zhang, Z., Jiang, Q., Jiang, F., Ding, G., Zhang, R., Wang, L., Zhang, L., Robin, A.M., Katakowski, M., Chopp, M., 2004. In vivo magnetic resonance imaging tracks adult neural progenitor cell targeting of brain tumor. Neuroimage. 23, 281–287.
Zurkiya, O., Hu, X., 2006. Off-resonance saturation as a means of generating contrast with superparamagnetic nanoparticles. Magn Reson Med 56, 726–732.
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Shapiro, E.M., Koretsky, A.P. (2008). Micron-Sized Iron Oxide Particles (MPIOs) for Cellular Imaging: More Bang for the Buck. In: Bulte, J.W., Modo, M.M. (eds) Nanoparticles in Biomedical Imaging. Fundamental Biomedical Technologies, vol 102. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72027-2_7
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DOI: https://doi.org/10.1007/978-0-387-72027-2_7
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