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Applications of Second Generation Sequencing Technologies in Complex Disorders

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Behavioral Neurogenetics

Part of the book series: Current Topics in Behavioral Neurosciences ((CTBN,volume 12))

Abstract

Second generation sequencing (2ndGS) technologies generate unprecedented amounts of sequence data very rapidly and at relatively limited costs, allowing the sequence of a human genome to be completed in a few weeks. The principle is on the basis of generating millions of relatively short reads from amplified single DNA fragments using iterative cycles of nucleotide extensions. However, the data generated on this scale present new challenges in interpretation, data analysis and data management. 2ndGS technologies are becoming widespread and are profoundly impacting biomedical research. Common applications include whole-genome sequencing, target resequencing, characterization of structural and copy number variation, profiling epigenetic modifications, transcriptome sequencing and identification of infectious agents. New methodologies and instruments that will enable to sequence the complete human genome in less than a day at a cost of less than $1,000 are currently in development.

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Authors and Affiliations

  1. Centro Nacional de Análisis Genómico, C/Baldiri Reixac 4, 08028, Barcelona, Spain

    Mònica Bayés, Simon Heath & Ivo Glynne Gut

Authors
  1. Mònica Bayés
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  2. Simon Heath
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  3. Ivo Glynne Gut
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Correspondence to Mònica Bayés .

Editor information

Editors and Affiliations

  1. , Dep of Pharmacology and Therapeutics, School of Pharmacy, College Rd, Cork, 30, Ireland

    John F. Cryan

  2. , Department of Psychiatry and Psychothera, University of Würzburg, Füchsleinstrasse 15, Würzburg, 97080, Germany

    Andreas Reif

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© 2011 Springer-Verlag Berlin Heidelberg

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Bayés, M., Heath, S., Gut, I.G. (2011). Applications of Second Generation Sequencing Technologies in Complex Disorders. In: Cryan, J., Reif, A. (eds) Behavioral Neurogenetics. Current Topics in Behavioral Neurosciences, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/7854_2011_196

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