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Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

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Fluorescent Methods to Study Biological Membranes

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 13))

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Abstract

This chapter describes the application of fluorescence correlation spectroscopy (FCS) as a powerful technique for the study of membrane organization and interactions. Monitoring the fluorescence signal fluctuations allows resolving concentrations, diffusion coefficients, and binding of several membrane components in experiments in vitro as well as in vivo.

We discuss the basic principles of FCS and explain novel implementations of FCS introduced to overcome the technical difficulties present in the standard version of fluorescence correlation spectroscopy. Finally, we report several examples of studies with the application of FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization and membrane interactions.

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Authors and Affiliations

  1. Max Planck Institute for Intelligent Systems and German Cancer Research Center, Bioquant, Im Neuenheimer Feld 267, Heidelberg, 69120, Germany

    Monika Zelman-Femiak, Yamunadevi Subburaj & Ana J. García-Sáez

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  1. Monika Zelman-Femiak
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  2. Yamunadevi Subburaj
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  3. Ana J. García-Sáez
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Correspondence to Ana J. García-Sáez .

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Editors and Affiliations

  1. Faculté de Pharmacie, Laboratoire de Biophotonique, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Yves Mély

  2. Faculté de Pharmacie, Laboratoire de Biophotonique et, Université de Strasbourg, route du Rhin 74, Illkirch, 67401, France

    Guy Duportail

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Zelman-Femiak, M., Subburaj, Y., García-Sáez, A.J. (2012). Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions. In: Mély, Y., Duportail, G. (eds) Fluorescent Methods to Study Biological Membranes. Springer Series on Fluorescence, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2012_49

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