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Investigating the Life Cycle of HIV with Fluorescent Proteins

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Fluorescent Proteins II

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 12))

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Abstract

Sensitive fluorescence methods based on fluorescent proteins (FPs) are being widely applied in the life sciences. Virology is no exception and the entry, assembly, and release of individual viruses are being investigated in living cells with unprecedented temporal and spatial resolution. In this chapter, we will focus on how FPs are used to explore the interaction of the human immunodeficiency virus (HIV) with its host cell. The first challenge is to fluorescently label the virion without interfering with virus functionality. Once components of the virus are labeled, processes such as entry, assembly, and budding can be directly followed optically in live cells in real time. Using single virus tracing, the interaction of individual viruses with the plasma membrane, virion uptake, as well as the kinetics of HIV assembly and release have been investigated. Furthermore, a variety of fluorescence microscopy techniques, e.g., Förster resonance energy transfer or image correlation microscopy, as well as FPs with special properties, e.g., photoconvertible proteins, offer new means of gaining mechanistic insight into processes involved in HIV replication.

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Authors and Affiliations

  1. Physical Chemistry, Department of Chemistry and Biochemistry, Ludwig-Maximilians University, Butenandtstraße 11, 81377, Munich, Germany

    Viola Baumgärtel, Sergey Ivanchenko & Don C. Lamb

  2. Department of Infectious Diseases, Virology, University Hospital Heidelberg, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany

    Barbara Müller

  3. Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Don C. Lamb

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  1. Viola Baumgärtel
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  2. Sergey Ivanchenko
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  3. Barbara Müller
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  4. Don C. Lamb
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Correspondence to Barbara Müller or Don C. Lamb .

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  1. , Campus B2 2, Saarland University, Saarbrücken, 66123, Saarland, Germany

    Gregor Jung

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Baumgärtel, V., Ivanchenko, S., Müller, B., Lamb, D.C. (2011). Investigating the Life Cycle of HIV with Fluorescent Proteins. In: Jung, G. (eds) Fluorescent Proteins II. Springer Series on Fluorescence, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2011_32

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