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Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues

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Standardization and Quality Assurance in Fluorescence Measurements II

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 6))

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Abstract

A short overview on principles and applications of fluorescence measurements is given, and three methods are described in detail. These methods include total internal reflection fluorescence (TIRF) microscopy and screening, fluorescence anisotropy as well as Förster resonance energy transfer (FRET) and total internal reflection energy transfer (TIRET) microscopy. All these methods have a high potential in biomedical research and in vitro diagnostics, but also need further optimization and standardization. The present chapter shows some approaches towards those goals and describes possible applications.

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Authors and Affiliations

  1. Hochschule Aalen, Institut für Angewandte Forschung, Anton-Huber-Str. 21, 73430, Aalen, Germany

    Herbert Schneckenburger

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  1. Herbert Schneckenburger
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Correspondence to Herbert Schneckenburger .

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Ute Resch-Genger

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© 2008 Springer-Verlag Berlin Heidelberg

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Schneckenburger, H. (2008). Fluorescence Techniques in Biomedical Diagnostics: Instrumentation, Analysis and Unresolved Issues. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_045

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