Summary
Resemblances and differences on photosynthesis of C3 plant and cyanobacterium Synechococcus PCC7942 were discussed with a simplified model of photosynthesis and photorespiration. Then the transformation of the cyanobacterium was shown with Chromatium vinosum (Cv) RuBisCO. Total RuBisCO activity and photosynthesis were compared with wild type S. 7942 and RuBisCO high expression mutant. Results were explained with RuBisCO environment of S. 7942 mutant. For excluding the influence of inorganic carbon concentrating mechanism (CCM) on the effect of RuBisCO, carboxysome less mutant was created with homologous recombination of ccm operon to be replaced with kanamycine resistant gene. And the deletion of the operon was confirmed by genomic PCR on the transformant. The confirmation was performed with three primers. Electron micrograms of S. 7942 show the existence of carboxysome in wild type and its absence in the mutant. Growth rate of CL mutant on different CO2 concentration shows that there is a possibility to increase a photosynthetic ability of a cyanobacterium with increases the RuBisCO activity.
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References
Caemmerer S von, Edmondson DL (1986) Relationship between steady-state gas exchange, in vivo ribulose bisphosphate carboxylase activity and intermediates in Raphanus sativus. Aust J Plant Physiol 13: 69–688
Haranoh K, Inoue N, Kojima K, Satoh R, Nishino T, Wada K, Ihara H, Tsuyama S, Kobayashi H, Iwaki T, Wadano A. Expression of foreign type ribulose 1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) stimulate photosynthesis in cyanobacterium Synechococcus PCC7942 cells, in preparation
Kotani H, Kaneko T, Matsubayashi T, Sato S, Sugiura M, Tabata S (1994) A physical map of the genome of a unicellular cyanobacterium Synechocystis sp. strain PCC6803. DNA Res 1:303–307
Orkwiszewski KG, Kaney AR (1974) Genetic transformation of the blue-green bacterium, Anacystis nidulans. Arch Mikrobiol 98:31–37
Robinson AL, Rhodes JS, Keith DW (2003) Assessment of potential carbon dioxide reductions due to biomass-coal cofiring in the United States. Environ Sci Technol 37:5081–9
Satoh R (1998) Analysis of ribulose bisphosphate carboxylase/oxygenase activity in a cyanobacterium Synechococcus PCC7942, in PhD thesis
Shibata M, Katoh H, Sonoda M, Ohkawa H, Shimoyama M, Fukuzawa H, Kaplan A, Ogawa T (2002) Genes essential to sodium-dependent bicarbonate transport in cyanobacteria: function and phylogenetic analysis. J Biol Chem 277:18658–18664
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© 2005 Springer-Verlag Tokyo
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Wadano, A., Tsukamoto, M., Nakano, Y., Iwaki, T. (2005). Modification of CO2 fixation of photosynthetic prokaryote. In: Omasa, K., Nouchi, I., De Kok, L.J. (eds) Plant Responses to Air Pollution and Global Change. Springer, Tokyo. https://doi.org/10.1007/4-431-31014-2_17
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DOI: https://doi.org/10.1007/4-431-31014-2_17
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-31013-6
Online ISBN: 978-4-431-31014-3
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