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Flow cytometry

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Principles of Immunopharmacology

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Summary

Flow cytometry is a powerful technique for analyzing cells in suspension, and has been extensively applied to the analysis of lymphocytes and their subsets. Flow cytometric assays can be divided into IMMUNOPHENOTYPING assays and functional assays, the latter requiring in vitro stimulation of cells in order to read out a response, such as CYTOKINE production. Such assays are being applied to the monitoring of clinical disease states and responses to vaccination or immunomodulation, and they have great potential to be used in measuring immunotoxicology as well.

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Author information

Authors and Affiliations

  1. BD Biosciences, 2350 Qume Drive, San Jose, CA, 95131, USA

    John F. Dunne & Holden T. Maecker

Authors
  1. John F. Dunne
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  2. Holden T. Maecker
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Editor information

Editors and Affiliations

  1. Faculty of Pharmacy, Department of Pharmacology and Pathophysiology, Sorbonnelaan 16, 3584 CA, Utrecht, The Netherlands

    Frans P. Nijkamp

  2. PLIVA Research Institute Ltd., Prilaz baruna Filipovića 29, HR-10 000, Zagreb, Croatia

    Michael J. Parnham

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© 2005 Birkhäuser Verlag

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Dunne, J.F., Maecker, H.T. (2005). Flow cytometry. In: Nijkamp, F.P., Parnham, M.J. (eds) Principles of Immunopharmacology. Birkhäuser Basel. https://doi.org/10.1007/3-7643-7408-X_13

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