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MALDI-ToF Mass Spectrometry for Studying Noncovalent Complexes of Biomolecules

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Applications of MALDI-TOF Spectroscopy

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 331))

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been demonstrated to be a valuable tool to investigate noncovalent interactions of biomolecules. The direct detection of noncovalent assemblies is often more troublesome than with electrospray ionization. Using dedicated sample preparation techniques and carefully optimized instrumental parameters, a number of biomolecule assemblies were successfully analyzed. For complexes dissociating under MALDI conditions, covalent stabilization with chemical cross-linking is a suitable alternative. Indirect methods allow the detection of noncovalent assemblies by monitoring the fading of binding partners or altered H/D exchange patterns.

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Abbreviations

ACN:

Acetonitrile

ADP:

Adenosine-5′-diphosphate

AMNP:

2-Amino-4-methyl-5-nitropyridine

AMP:

Adenosine-5′-monophosphate

ANA:

2-Aminonicotinic acid

AP:

Atmospheric pressure

ATP:

Adenosine-5′-triphosphate

ATT:

6-Aza-2-thiothymine

CHCA:

α-Cyano-4-hydroxycinnamic acid

DHAP:

Dihydroxyacetophenone

DHB:

Dihydroxybenzoic acid

DNA:

Deoxyribonucleic acid

ESI:

Electrospray ionization

FA:

Ferulic acid

hERα LBD:

Human estrogen receptor α ligand binding domain

HIV:

Human immunodeficiency virus

HPA:

3-Hydroxypicolinic acid

IR:

Infrared

iTRAQ:

Isobaric tag for relative and absolute quantitation

K a :

Association constant

K d :

Dissociation constant

LILBID:

Laser induced liquid beam or bead ionization/desorption

MALDI:

Matrix-assisted laser desorption/ionization

MCP:

Microchannel plate detector

MS:

Mass spectrometry

NHS:

N-Hydroxysuccinimide

PNA:

p-Nitroaniline

RNA:

Ribonucleic acid

SA:

Sinapinic acid

TFA:

Trifluoroacetic acid

THAP:

Trihydroxyacetophenone

THF:

Tetrahydrofuran

ToF:

Time-of-flight

TrpR:

Tryptophan repressor

UV:

Ultraviolet

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Acknowledgments

We would like to thank Richard Caprioli and Dobrin Nedelkov for providing original versions of figures. Financial support for this work from the Swiss National Science Foundation (grant no. 200020_124663) is gratefully acknowledged.

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  1. Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland

    Stefanie Mädler, Elisabetta Boeri Erba & Renato Zenobi

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  1. Stefanie Mädler
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Correspondence to Renato Zenobi .

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  1. , Department of Chemistry, Hong Kong Baptist University, Ho Sin Hang Campus, Hong Kong, 000000000, China, People's Republic

    Zongwei Cai

  2. , Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 000000000, China, People's Republic

    Shuying Liu

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Mädler, S., Erba, E.B., Zenobi, R. (2012). MALDI-ToF Mass Spectrometry for Studying Noncovalent Complexes of Biomolecules. In: Cai, Z., Liu, S. (eds) Applications of MALDI-TOF Spectroscopy. Topics in Current Chemistry, vol 331. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_311

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