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Lipid Bilayer Membrane Arrays: Fabrication and Applications

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Future Trends in Biotechnology

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 131))

Abstract

The lipid bilayer is one of the most important self-assembled structures in nature. In addition to compartmentalizing the cell, the lipid bilayer maintains many physical and biological characteristics of cell membranes, including lateral fluidity. It provides a wealth of opportunities for the study of membrane properties. Recently there has been an increasing interest in lipid bilayer arrays fabrication and their applications. In this review, the leading methods for creating lipid bilayer arrays are categorized as mechanical methods, pre-patterning substrate, direct UV patterning, direct blotting or stamping, polymer liftoff technique, robotic micro spotting, and microfluidics. The applications of bilayer arrays for cell adhesion and activation, lipid bilayer based 2D electrophoresis, and high-throughput binding assays are also presented.

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Abbreviations

TR-PE:

Texas Red 1, 2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine

D-291:

4-(4-(didecylamino) styryl)-N-methylpyridinium iodide (4-di-10-ASP)

NBD-PE:

(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt)

Egg PC:

Egg phosphatidylcholine

POPC:

1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine

NBD-PG:

1-Acyl-2-{12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl}-sn-glycero-3-[phospho-rac-(1-glycerol)] (ammonium salt)

TR-BSA:

Bovine serum albumin labeled with Texas Red X-SE

Rh-PE:

1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine-rhodamine B sulfonyl)

DOPC:

1,2-dioleoyl-sn-glycero-3-phosphocholine

Oregon Green-DHPE:

Oregon Green labeled 1,2-dihexadecanoyl-sn-glycero-3- phosphoethanolamine

Marina Blue-DHPE:

Marina Blue labeled 1,2-dihexadecanoyl-sn-glycero-3- phosphoethanolamine

DNP-cap-DPPE:

1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (ammonium salt)

GPI:

Glycan-phosphatidyl inositol

PG:

Phosphatidylglycerol

PC:

Phosphatidylcholine

PS:

Phosphatidylserine

DPPC:

1,2-dipalmitoyl-sn-glycero-3-phosphocholine

NBD PS:

1,2-dioleoyl-sn-glycero-3-phospho-l-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (diammonium salt)

FITC:

Fluorescein isothiocyanate

DOTAP:

1,2-dioleoyl-3-trimethylammoniumpropane (chloride salt)

Biotin-cap DOPE:

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt)

Tm :

Phase transition temperature

SAM:

Self assembled monolayer

PDMS:

Poly(dimethyl siloxane)

AFM:

Atomic force microscopy

CFM:

Continuous flow microspotter

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 21003032) and the program for New Century Excellent Talents in University (Grant No. NCET-09-0054).

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  1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001, China

    Xiaojun Han, Guodong Qi, Xingtao Xu & Lei Wang

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Correspondence to Xiaojun Han .

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    Jian-Jiang Zhong

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Han, X., Qi, G., Xu, X., Wang, L. (2012). Lipid Bilayer Membrane Arrays: Fabrication and Applications. In: Zhong, JJ. (eds) Future Trends in Biotechnology. Advances in Biochemical Engineering/Biotechnology, vol 131. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2012_135

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