Abstract
Cellular imaging has achieved many new biological findings, among them GFP and other fluorescent proteins and small molecule based fluorescent sensors have been widely used, especially in the last decade. The design concept and application of chemical sensors are described, these being FRET based sensors and Zn2+ sensors.
Fluorescence resonance energy transfer (FRET) has been used extensively as the designing principle for fluorescent sensor molecules. One of the most significant advantages of designing sensor molecules with FRET modulation is that it can enable ratiometric measurement in living cells, which reduces the artifact from microscopic imaging systems. The design strategy for the development of small molecular FRET sensors is described in terms of avoiding close contact of donor fluorophore and acceptor fluorophore in aqueous solution. Furthermore, a strategy to design FRET sensors with modulating overlap integrals of donor and acceptor is introduced.
Numerous tools for Zn2+ sensing in living cells have become available in the last 8 years. Among them, fluorescence imaging using fluorescent sensor molecules has been the most popular approach. Some of these sensor molecules can be used to visualize Zn2+ in living cells. Some of the biological functions of Zn2+ were clarified using these sensor molecules, especially in neuronal cells, which contain a high concentration of free Zn2 +.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tsien RY (1994) Chem Eng News 72:34
Czarnik AW (1995) Chem Biol 2:423
Rurack K, Resch-Genger U (2002) Chem Soc Rev 31:116
Grynkiewicz G, Poenie M, Tsien RY (1985) J Biol Chem 260:3440
Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY (1997) Nature 388:882
Kojima H, Nakatsubo N, Kikuchi K, Kawahara S, Kirino Y, Nagoshi H, Hirata Y, Nagano T (1998) Anal Chem 70:2446
Hirano T, Kikuchi K, Urano Y, Higuchi T, Nagano T (2000) J Am Chem Soc 122:12399
Tsien RY, Harootunian AT (1990) Cell Calcium 11:93
Minta A, Tsien RY (1989) J Biol Chem 264:19449
Mizukami S, Nagano T, Urano Y, Odani A, Kikuchi K (2002) J Am Chem Soc 124:3920
Maruyama S, Kikuchi K, Hirano T, Urano Y, Nagano T (2002) J Am Chem Soc 124:10650
Lakowicz JR (1999) Principles of fluorescence spectroscopy. Kluwer, New York
Adams SR, Harootunian AT, Buechler YJ, Taylor SS, Tsien RY (1991) Nature 349:694
Mochizuki N, Yamashita S, Kurokawa K, Ohba Y, Nagai T, Miyawaki A, Matsuda M (2001) Nature 411:1065
Zlokarnik G, Negulescu PA, Knapp TE, Mere L, Burres N, Feng L, Whitney M, Roemer K, Tsien RY (1998) Science 279:84
Gonzalez JE, Tsien RY (1995) Biophys J 69:1272
Kawanishi Y, Kikuchi K, Takakusa H, Mizukami S, Urano Y, Higuchi T, Nagano T (2000) Angew Chem Int Ed 39:3438
Takakusa H, Kikuchi K, Urano Y, Sakamoto S, Yamaguchi K, Nagano T (2002) J Am Chem Soc 124:1653
Takakusa H, Kikuchi K, Urano Y, Higuchi T, Nagano T (2001) Anal Chem 73:939
Mizukami S, Kikuchi K, Higuchi T, Urano Y, Mashima T, Tsuruo T, Nagano T (1999) FEBS Lett 453:356
Gurtu V, Kain SR, Zhang G (1997) Anal Biochem 25:198
Tewari M, Quan LT, O’Rourke K, Desnoyers S, Zeng Z, Beidler DR, Poirier GG, Salvesen GS, Dixit VM (1995) Cell 81:801
Packard BZ, Toptygin DD, Komoriya A, Brand L (1997) Biophys Chem 67:167
Daugherty DL, Gellman SH (1999) J Am Chem Soc 121:4325
Geoghegan KL, Rosner PJ, Hoth LR (2000) Bioconjug Chem 11:71
Valdes-Aguilera O, Neckers DC (1989) Acc Chem Res 22:171
West WS, Pearce J (1965) Phys Chem 69:1894
Li Y, Glazer AN (1999) Bioconjug Chem 10:241
Sträter N, Lipscomb WN, Klabunde T, Krebs B (1996) Angew Chem Int Ed 35:2024
Bollen M, Gijsbers R, Ceulemans H, Stalmans W, Stefan C (2000) Crit Rev Biochem Mol 35:393
Zimmermann H (1999) Trends Pharmacol Sci 20:231
Goding JW, Terkeltaub R, Maurice M, Deterre P, Sali A, Belli SI (1998) Immunol Rev 161:11
Clair T, Lee HY, Liotta LA, Stracke ML (1997) J Biol Chem 272:996
Berkessel A, Riedl R (1997) Angew Chem Int Ed 36:1481
Takakusa H, Kikuchi K, Urano Y, Kojima H, Nagano T (2003) Chem Eur J 9:1479
Majeti R, Weiss A (2001) Chem Rev 101:2441
Wang QP, Scheigetz J, Gilbert M, Snider J, Ramachandran C (1999) Biochim Biophys Acta 1431:14
Zalewski PD, Forbes IJ, Betts WH (1993) Biochem J 296:403–408
Ueno S, Tsukamoto M, Hirano T, Kikuchi K, Yamada MK, Nishiyama M, Nagano T, Matsuki N, Ikegaya Y (2002) J Cell Biol 158:215–220
Sensi SL, Ton-That D, Sullivan PG, Jonas EA, Gee KR, Kaczmarek LK, Weiss JH (2003) Proc Natl Acad Sci U S A 100:6157–6162
da Silva JJRF, Williams RJP (2001) The biological chemistry of elements: the inorganic chemistry of life, 2nd edn. Oxford University Press, New York pp 315–339
Zalewski PD, Jian X, Soon LLL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ (1996) Reprod Fertil Dev 8:1097–1105
Weiss JH, Sensi SL, Koh JY (2000) Trends Pharm Sci 21:395–401
Hirano T, Kikuchi K, Urano Y, Higuchi T, Nagano T (2000) Angew Chem Int Ed 39:1052–1054
Hirano T, Kikuchi K, Urano Y, Higuchi T, Nagano T (2000) J Am Chem Soc 122:12399–12400
Hirano T, Kikuchi K, Urano Y, Nagano T (2002) J Am Chem Soc 124:6555–6562
Lee YV, Hough CJ, Sarvey JM (2003) Sci STKE:pe19
Komatsu K, Kikuchi K, Urano Y, Kojima H, Nagano T (2005) J Am Chem Soc 127:10197
Kawabata E, Kikuchi K, Urano Y, Kojima H, Odani A, Nagano T (2005) J Am Chem Soc 127:818
Maruyama S, Kikuchi K, Hirano T, Urano Y, Nagano T (2002) J Am Chem Soc 124:10650
Dineley KE, Malaiyandi LM, Reynolds IJ (2002) Mol Pharmacol 62:618
MacDiarmid CW, Milanick MA, Eide DJ (2003) J Biol Chem 278:15065
Henary MM, Fahrni CJ (2002) J Phys Chem A 106:5210
Fahrni CJ, Henary MM, VanDerveer DG (2002) J Phys Chem A 106:7655
Henary MM, Wu Y, Fahrni CJ (2004) Chem Eur J 10:3015
Taki M, Wolford JL, O’Halloran TV (2004) J Am Chem Soc 126:712
Hanaoka K, Kikuchi K, Kojima H, Urano Y, Nagano T (2003) Angew Chem Int Ed 42:2996
Hanaoka K, Kikuchi K, Kojima H, Urano Y, Nagano T (2004) J Am Chem Soc 126:12470
Hanaoka K, Kikuchi K, Kobayashi S, Nagano T (2007) J Am Chem Soc 128:13502
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Kikuchi, K. (2009). Design, Synthesis, and Biological Application of Fluorescent Sensor Molecules for Cellular Imaging. In: Endo, I., Nagamune, T. (eds) Nano/Micro Biotechnology. Advances in Biochemical Engineering / Biotechnology, vol 119. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_42
Download citation
DOI: https://doi.org/10.1007/10_2008_42
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-14946-7
Online ISBN: 978-3-642-14947-4
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)