Skip to main content

Abstract

Mutants are central to elucidating gene function in many plant species. A range of symbiotic and other mutants have been created in legumes using both gamma (γ-) irradiation and chemical mutagenesis. Advantages and disadvantages of these two methods are presented, along with an outline of procedures for and indicators of successful mutagenesis in L. japonicus. Screening procedures for symbiotic mutants, tannins and starch mutants are also described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Barratt DHP, Barber L, Kruger NJ, Smith AM, Wang TL, and Martin C. (2001) Multiple, distinct isoforms of sucrose synthase in pea. Plant Physiology 127, 655–664.

    Article  CAS  PubMed  Google Scholar 

  • Blixt S. (1972) Mutation genetics in pea. Agri Hortique Genetica, 1–293.

    Google Scholar 

  • Bonfante P, Genre A, Faccio A, Martini I, Schauser L, Stougaard J, Webb J, and Parniske M. (2000) The Lotus japonicus LjSym4 gene is required for the successful symbiotic infection of root epidermal cells. Molecular Plant-Microbe Interactions 13, 1109–1120.

    CAS  PubMed  Google Scholar 

  • Carroll BJ, McNeil DL, and Gresshoff PM. (1986) Mutagenesis of soybean (Glycine max (L) MERR.) and the isolation of non-nodulating mutants. Plant Science 47, 109–114.

    Article  Google Scholar 

  • Duc G and Messager A. (1989) Mutagenesis of pea (Pisum sativum L.) and the isolation of mutants for nodulation and nitrogen fixation. Plant Science 60, 207–213.

    Google Scholar 

  • Harrison C, Hedley CL, and Wang TL. (1998) Evidence that the rug3 locus of pea (Pisum sativum L.) encodes plastidial phosphoglucomutase confirms that the imported substrate for starch synthesis in pea amyloplasts is glucose6phosphate. The Plant Journal 13, 753–7562.

    Article  CAS  Google Scholar 

  • Imaizumi-Anraku H. (2003) Legume nodulation and mycorrhiza formation-common genes are involved in both symbiotic interactions. Nippon Nogeikagaku Kaishi-Journal of the Japan Society for Bioscience Biotechnology and Agrochemistry 77, 121–123.

    CAS  Google Scholar 

  • Imaizumi-Anraku H, Kawaguchi M, Koiwa H, Akao S, and Syono, K. (1997) Two ineffective-nodulating mutants of Lotus japonicus — Different phenotypes caused by the blockage of endocytotic bacterial release and nodule maturation. Plant and Cell Physiology 38, 871–881.

    CAS  Google Scholar 

  • Kneen BE and Larue, TA. (1988) Induced Symbiosis Mutants of Pea (Pisum-Sativum) and Sweetclover (Melilotus-Alba-Annua). Plant Science 58, 177–182.

    Article  Google Scholar 

  • Krusell L, Madsen LH, Sato S, Aubert G, Genua A, Szczyglowski K, Duc G, Kaneko T, Tabata S, de Bruijn F, Pajuelo E, Sandal N, and Stougaard J. (2002) Shoot control of root development and nodulation is mediated by a receptor-like kinase. Nature 420, 422–426.

    Article  CAS  PubMed  Google Scholar 

  • Li Y-G, Tanner GJ, and Larkin PJ. (1996) The DMACA-HCl protocol and the threshold proanthocyanidin content for bloat-safety in forage legumes. Journal of Science, Food and Agriculture 70, 89–101.

    CAS  Google Scholar 

  • Márquez AJ, Betti M, García-Calderón M, Pal’ove-Balang P, Díaz P, Monza J (2005) Nitrate assimilation in Lotus japonicus. Journal of Experimental Botany (in press).

    Google Scholar 

  • Morandi D, Sagan M, Prado-Vivant E, and Duc G. (2000) Influence of genes determining supernodulation on root colonization by the mycorrhizal fungus Glomus mosseae in Pisum sativum and Medicago truncatula mutants. Mycorrhiza 10, 37–42.

    Article  CAS  Google Scholar 

  • Nakata PA, and McConn MM. (2000) Isolation of Medicago truncatula mutants defective in calcium oxalate crystal formation. Plant Physiology 124, 1097–1104.

    Article  CAS  PubMed  Google Scholar 

  • Orea A (1999) Nitrate assimilation in the model legume Lotus japonicus. Ph. D. Thesis, University of Seville (Spain).

    Google Scholar 

  • Orea A, Pajuelo P, Pajuelo E, Quidiello C, Romero JM, and Márquez AJ. (2002) Isolation of photorespiratory mutants from Lotus japonicus deficient in glutamine synthetase. Physiologia Plantarum 115, 352–361.

    Article  CAS  PubMed  Google Scholar 

  • Perry JA, Wang TL, Welham TJ, Gardner S, Pike JM, Yoshida S and Parniske M. (2003) A TILLING Reverse Genetics Tool and a Web-Accessible Collection of Mutants of the Legume Lotus japonicus. Plant Physiology 131, 866–871.

    Article  CAS  PubMed  Google Scholar 

  • Sagan M, Ney B, and Duc G. (1993) Plant Symbiotic Mutants as a Tool to Analyze Nitrogen Nutrition and Yield Relationship in Field-Grown Peas (Pisum-Sativum L). Plant and Soil 153, 33–45.

    CAS  Google Scholar 

  • Schauser L, Handberg K, Sandal N, Stiller J, Thykjaer T, Pajuelo E, Nielsen A, and Stougaard J. (1998) Symbiotic mutants deficient in nodule establishment identified after T-DNA transformation of Lotus japonicus. Molecular and General Genetics 259, 414–423.

    CAS  PubMed  Google Scholar 

  • Schauser L, Roussis A, Stiller J, and Stougaard J. (1999) A plant regulator controlling development of symbiotic root nodules. Nature 402, 191–195.

    CAS  PubMed  Google Scholar 

  • Schreck S. (2002) The role of plastidial phosphoglucomutase in carbon partitioning. PhD thesis. University of East Anglia UK.

    Google Scholar 

  • Senoo K, Solaiman MZ, Kawaguchi M, Imaizumi-Anraku H, Akao S, Tanaka A, and Obata H. (2000) Isolation of two different phenotypes of mycorrhizal mutants in the model legume plant Lotus japonicus after EMS-treatment. Plant and Cell Physiology 41, 726–732.

    CAS  PubMed  Google Scholar 

  • Sharma SK and Sharma B. (1986) Mutagen sensitivity and mutability in lentil. Theoretical and Applied Genetics 71, 820–825.

    Google Scholar 

  • Skadhauge B. (1996) Genetics and Biochemistry of proanthocyanidin biosynthesis and their biological significance in crop plants. PhD thesis. The Royal Vetinary and Agriculture University, Copenhagen, Denmark.

    Google Scholar 

  • Stafford HA, and Cheng T-Y. (1980) The procyanidins of douglas fir seedlings, callus and cell suspension cultures derived from cotyledons. Phytochemistry 19, 131–135.

    Article  CAS  Google Scholar 

  • Stracke S, Kistner C, Yoshida S, Mulder L, Sato S, Kaneko T, Tabata S, Sandal N, Stougaard J, Szczyglowski K, and Parniske M. (2002) A plant receptor-like kinase required for both bacterial and fungal symbiosis. Nature 417, 959–962.

    Article  CAS  PubMed  Google Scholar 

  • Szczyglowski K, Shaw RS, Wopereis J, Copeland S, Hamburger D, Kasiborski B, Dazzo FB, and de Bruijn FJ. (1998) Nodule organogenesis and symbiotic mutants of the model legume Lotus japonicus. Molecular Plant-Microbe Interactions 11, 684–697.

    CAS  Google Scholar 

  • Vincent J. (1970) A Manual for the Practical Study of Root-nodule Bacteria. Blackwell Scientific Publications, Oxford and Edinburgh, 164.

    Google Scholar 

  • Wang TL, Hadavizideh A, Harwood A, Welham TJ, Harwood WA, Faulks R, and Hedley CL. (1990) An Analysis of Seed Development. In:Pisum sativum XIII The Chemical Induction of Storage Product Mutants. Plant Breeding 105, 311–320.

    CAS  Google Scholar 

  • Wegel E, Schauser L, Sandal N, Stougaard J, and Parniske M. (1998) Mycorrhiza mutants of Lotus japonicus define genetically independent steps during symbiotic infection. Molecular Plant-Microbe Interactions 11, 933–936.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to K Judith Webb .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Springer

About this chapter

Cite this chapter

Webb, K.J., Robbins, M., Wang, T.L., Parniske, M., Márquez, A. (2005). Mutagenesis. In: Márquez, A.J. (eds) Lotus japonicus Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3735-X_18

Download citation

Publish with us

Policies and ethics