Abstract
Continuous cell lines are not capable of complete oxidising glucose via the TCA cycle. Recently, we presented a strategy of inverse metabolic engineering to increase the glycolytic flux into the TCA. This work summarises the effect of yeast pyruvate carboxylase 2 (PYC2) expressed in the cytoplasm of different host systems: BHK-21, CHO-K1, CHO-DG44, HEK293 and BTI-Tn-5B1-4 (High Five™) cells.
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Bollatifogolin, M. et al. (2005). Impact of Yeast Pyruvate Carboxylase on the Productivity of Animal Host Cell Lines. In: Gòdia, F., Fussenegger, M. (eds) Animal Cell Technology Meets Genomics. ESACT Proceedings, vol 2. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3103-3_13
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DOI: https://doi.org/10.1007/1-4020-3103-3_13
Publisher Name: Springer, Dordrecht
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