Abstract
It has been widely reported that metabolism, cell growth, cell density, product secretion and specific antibody productivity in mammalian cells is strongly affected by osmotic conditions. However, because hyper- and hypo-osmotic pressure suppresses cell growth, the enhanced final product concentration of the culture is not observed. Therefore by understanding the basic cellular processes of a GS-NS0 mammalian cell culture system would not only assist in the design of a more efficient mammalian cell culture systems but it will also aid the optimization of the production. Various properties of mammalian culture systems, such as, productivity, cell viability, metabolism, ion balance and the genes regulated during the culture of the GS-NS0 system under osmotic pressure of 210, 290, 370 and 450 mOsm/kg have been identified, and it is shown that there is a decrease in the growth rate of hyper- and hypo-osmotic cultures. Further differences have been identified in calcium accumulation, metabolism of glucose, glutamate and lactate. Additionally it is shown that there are over 600 genes involved in ion transport, accumulation of osmolytes, cell cycle distribution, proliferation, cytoskeletal organization and cell metabolism that are regulated by the changes in osmotic pressure.
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Wu, MH., Dimopoulos, G., Mantalaris, A., Varley, J. (2005). Effect of Osmotic Pressure on Gs-Ns0 Expression System. In: Gòdia, F., Fussenegger, M. (eds) Animal Cell Technology Meets Genomics. ESACT Proceedings, vol 2. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3103-3_1
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DOI: https://doi.org/10.1007/1-4020-3103-3_1
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